Enhancing the expression level of human epidermal growth factor using the polyhedrin protein sequence of BmNPV.
- Author:
Yuedong LI
1
;
Xingyang WANG
1
;
Shuohao LI
1
;
Xiaofeng WU
1
Author Information
- Publication Type:Journal Article
- Keywords: baculovirus; fusion expression; polyhedrin; silkworm
- MeSH: Humans; Epidermal Growth Factor/biosynthesis*; Genetic Vectors/genetics*; Recombinant Fusion Proteins/biosynthesis*; Animals; Bombyx/metabolism*; Occlusion Body Matrix Proteins/genetics*; Nucleopolyhedroviruses/genetics*; Amino Acid Sequence
- From: Chinese Journal of Biotechnology 2024;40(11):4211-4218
- CountryChina
- Language:Chinese
- Abstract: Human epidermal growth factor (hEGF) can be applied in the treatment of surgical trauma (burns, scalds), tissue repair, skin moisturizing, beauty, skincare, etc. However, the low expression and high cost limit the application of hEGF. In order to improve the expression level of hEGF and reduce the production cost, considering the high expression of polyhedrin, this study fused a partial sequence of polyhedrin with hEGF and expressed the fused sequence by using a silkworm baculovirus expression vector system. In view of the small molecular weight of hEGF, we connected hEGF genes in series and optimized the codons to construct multiple fusion expression vectors by fusing different partial sequences of polyhedrin at the N-terminus. The results showed that through the above strategy, the protein expression level of hEGF was significantly increased. The expression vector containing three concatenated hEGF genes with optimized codons and fused with the sequence encoding 25 or 35 residues at the N-terminus of polyhedrin showed the highest expression level.
