Development of a tumor organoid culture system with peptide-based hydrogels.

Huibin WANG; Dongdong ZHAO; Lu ZHANG; Zhandong WEI; Jun LIANG; Changhao BI

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Development of a tumor organoid culture system with peptide-based hydrogels.

Author: Huibin WANG ¹ ; Dongdong ZHAO ² ; Lu ZHANG ² ; Zhandong WEI ² ; Jun LIANG ¹ ; Changhao BI ²
Author Information

1. College of Light Industry Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Publication Type:Journal Article
Keywords: 3D culture; organoid; peptide-based hydrogel; self-assembly; tumor
MeSH: Hydrogels/chemistry*; Organoids; Humans; Peptides/pharmacology*; Cell Line, Tumor; Cell Culture Techniques, Three Dimensional; Cell Culture Techniques; Cell Survival/drug effects*; Nanofibers/chemistry*
From: Chinese Journal of Biotechnology 2024;40(11):4157-4170
CountryChina
Language:Chinese
Abstract: Peptide-based hydrogel, the polymer materials with a special network structure, are widely used in various fields of biomedicine due to their stable properties and biocompatibility. Environment-responsive self-assembled peptide aqueous solutions can respond to environment changes by the self-assembly of peptides into nanofiber networks. Peptide-based hydrogels well simulate the extracellular matrix and cell growth microenvironment, being suitable for 3D cell culture and organoid culture. To establish a tumor organoid culture system with peptide-based hydrogels, we cultured Panc-1, U87, and H358 cells in a 3D spherical manner using CulX Ⅱ peptide-based hydrogels in 24-well plates for 15 days. The organoids showed a 3D spherical shape, and their sizes increased with the extension of the culture time, with a final diameter ranging from 150 to 300 μm. The organoids had a large number, varying sizes, good cell viability, clear edges, and a good shape, which indicated successful organoid construction. The tumor organoid culture system established in this study with CulX Ⅱ peptide-based hydrogels provides a model for studying tumor pathogenesis, drug development, and tumor suppression.