Expression of influenza A H1N1 and H3N2 viruses Mosaic-HA1 antigens and evaluation of its immunogenicity in mice.

Fei HAN; Pengtao JIAO; Runshan LIN; Heqiao LI; Jianing MA; Hanzhong PEI; He ZHANG; Lei SUN; Tingrong LUO; Min ZHENG; Wenhui FAN; Wenjun LIU

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Expression of influenza A H1N1 and H3N2 viruses Mosaic-HA1 antigens and evaluation of its immunogenicity in mice.

Author: Fei HAN ¹ ; Pengtao JIAO ² ; Runshan LIN ² ; Heqiao LI ³ ; Jianing MA ² ; Hanzhong PEI ³ ; He ZHANG ³ ; Lei SUN ² ; Tingrong LUO ¹ ; Min ZHENG ³ ; Wenhui FAN ² ; Wenjun LIU ¹
Author Information

1. College of Animal Science and Technology, Guangxi University, Nanning 530004, Guangxi, China.
2. Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
3. Shenzhen Bay Laboratory, Shenzhen 518000, Guangdong, China.
Publication Type:Journal Article
Keywords: H1N1; H3N2; HA1 subunit; evaluation of immunogenicity; recombinant protein
MeSH: Animals; Influenza A Virus, H1N1 Subtype/genetics*; Influenza A Virus, H3N2 Subtype/genetics*; Mice, Inbred BALB C; Mice; Influenza Vaccines/genetics*; Hemagglutinin Glycoproteins, Influenza Virus/genetics*; Humans; Antibodies, Viral/blood*; Antigens, Viral/genetics*; Immunoglobulin G/immunology*; Female; Orthomyxoviridae Infections/prevention & control*; HEK293 Cells
From: Chinese Journal of Biotechnology 2024;40(11):4042-4056
CountryChina
Language:Chinese
Abstract: Vaccination is the most effective measure for reducing and preventing influenza and related complications. In this study, we analyzed the mutation trend and the antigen dominant site changes of the amino acid sequence of hemagglutinin subunit 1 (HA1) of human influenza A virus (IAV) in the northern hemisphere from 2012 to 2022. According to the HA1 sequences of A/Darwin/6/2021 (H3N2) and A/Wisconsin/588/2019 (H1N1) recommended by the World Health Organization in the 2022 influenza season in northern hemisphere, we employed the mosaic algorithm to design three Mosaic-HA1 antigens through stepwise substitution. Mosaic-HA1 was expressed and purified in 293F cells and then mixed with the alum adjuvant at a volume ratio of 1:1. The mixture was used to immunize BALB/c mice, and the immunogenicity was evaluated. Enzyme-linked immunosorbent assay showed that Mosaic-HA1 induced the production of IgG targeting two types of HA1, the specific IgG titers for binding to H3 protein and H1 protein reached 10⁵ and 10³ respectively. The challenge test showed that Mosaic-HA1 protected mice from H3N2 or H1N1. This study designs the vaccines by recombination of major antigenic sites in different subtypes of IAV, giving new insights into the development of multivalent subunit vaccines against influenza.