Preparation of polycaprolactone-polyethylene glycol-concentrated growth factor composite scaffolds and the effects on the biological properties of human periodontal ligament stem cells.
10.7518/hxkq.2025.2025044
- Author:
Li GAO
1
;
Mingyue ZHAO
1
;
Shun YANG
1
;
Runan WANG
1
;
Jiajia CHENG
1
;
Guangsheng CHEN
1
Author Information
1. School of Stomatology, Zunyi Medical University, Zunyi 563099, China.
- Publication Type:Journal Article
- Keywords:
cell proliferation;
concentrated growth factor;
human periodontal ligament stem cells;
osteogenic differentiation;
polycaprolactone-polyethylene glycol scaffold;
polycaprolactone-polyethylene glycol-concentrated growth factor composi-te scaffold
- MeSH:
Humans;
Polyesters/chemistry*;
Periodontal Ligament/cytology*;
Polyethylene Glycols/chemistry*;
Stem Cells/cytology*;
Tissue Scaffolds;
Cell Proliferation;
Osteogenesis;
Cell Differentiation;
Cell Adhesion;
Bone Morphogenetic Protein 2/metabolism*;
Cells, Cultured;
Alkaline Phosphatase/metabolism*;
Core Binding Factor Alpha 1 Subunit/metabolism*;
Intercellular Signaling Peptides and Proteins/pharmacology*;
Tissue Engineering/methods*
- From:
West China Journal of Stomatology
2025;43(6):819-828
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:This study investigated the effects of a polycaprolactone (PCL)-polyethylene glycol (PEG) scaffold incorporated with concentrated growth factor (CGF) on the adhesion, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs).
METHODS:The PCL-PEG-CGF composite scaffold was fabricated using an immersion and freeze-drying technique. Its microstructure, mechanical properties, and biocompatibility were systematically characterized. The hPDLSCs were isolated through enzymatic digestion, and the hPDLSCs were identified through flow cytometry. Third-passage hPDLSCs were seeded onto the composite scaffolds, and their adhesion, proliferation and osteogenic differentiation were assessed using CCK-8 assays, 4',6-diamidino-2-phenylindole (DAPI) staining, alkaline phosphatase (ALP) staining, alizarin red staining, and Western blot analysis of osteogenesis-related proteins [Runt-related transcription factor 2 (Runx2), ALP, and morphogenetic protein 2 (BMP2)].
RESULTS:Scanning electron microscopy revealed that the PCL-PEG-CGF composite scaffold exhibited a honeycomb-like structure with heterogeneous pore sizes. The composite scaffold exhibited excellent hydrophilicity, as evidenced by a contact angle (θ) approaching 0° within 6 s. Its elastic modulus was measured at (4.590 0±0.149 3) MPa, with comparable hydrophilicity, fracture tensile strength, and fracture elongation to PCL-PEG scaffold. The hPDLSCs exhibited significantly improved adhesion to the PCL-PEG-CGF composite scaffold compared with the PCL-PEG scaffold (P<0.01). Additionally, cell proliferation was markedly improved in all the experimental groups on days 3, 5, and 7 (P<0.01), and statistically significant differences were found between the PCL-PEG-CGF group and other groups (P<0.01). The PCL-PEG-CGF group showed significantly elevated ALP activity (P<0.05), increased mineralization nodule formation, and upregulated expression of osteogenic-related proteins (Runx2, BMP2 and ALP; P<0.05).
CONCLUSIONS:The PCL-PEG-CGF composite scaffold exhibited excellent mechanical properties and biocompatibility, enhancing the adhesion and proliferation of hPDLSCs and promoting their osteogenic differentiation by upregulating osteogenic-related proteins.
