Aloe-emodin inhibits scar tissue fibrosis through thrombospondin-1-PI3k-Akt pathway.
10.7518/hxkq.2025.2024422
- Author:
Hongbao GENG
1
;
Xingyi ZHANG
2
;
Siwei ZHOU
3
;
Na LI
4
;
Jia LIU
4
;
Xuewei YUAN
4
;
Chunliu NING
2
;
Xudong ZHANG
2
;
Wei HUANG
2
Author Information
1. Dept. of Stomatology, Women and Children's Health Hospital in Shijiazhuang, Shijiazhuang 050000, China.
2. Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Disease, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China.
3. Dept. of Stomatology, Shijiazhuang Fourth Hospital, Shijiazhuang 050011, China.
4. Dept. of Stomatology, Shijiazhuang Second Hospital, Shijiazhuang 050051, China.
- Publication Type:Journal Article
- Keywords:
PI3K-Akt signaling pathway;
aloe-emodin;
fibrosis;
palatal scar;
thrombospondin-1
- MeSH:
Thrombospondin 1/genetics*;
Humans;
Signal Transduction/drug effects*;
Fibroblasts/cytology*;
Proto-Oncogene Proteins c-akt/metabolism*;
Fibrosis;
Phosphatidylinositol 3-Kinases/metabolism*;
Cicatrix/metabolism*;
Cell Proliferation/drug effects*;
Anthraquinones/pharmacology*;
Cells, Cultured
- From:
West China Journal of Stomatology
2025;43(5):636-647
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To propose a hypothesis that aloe-emodin may inhibit scar tissue fibrosis through thrombospondin-1(THBS1)-PI3K-Akt pathway.
METHODS:By cultivating fibroblasts derived from scar tissue after cleft palate surgery in humans, aloe emodin of different concentrations (10, 20, 30, 40 and 50 μmol/L) was added to the cells which activity was detected. At the same time, transcriptome sequencing was performed on scar tissue and cells, and bioinformatics methods were used to explore potential targets and signaling pathways of scar tissue fibrosis.
RESULTS:Aloe-emodin had a concentration dependent inhibitory effect on fibroblast proliferation,with the 40 μmol/L concentration group showing the most significant effect. The results of tissue and cell sequencing indicated that differentially expressed genes were significantly enriched in extracellular matrix-receptor interaction pathway, and shared a common differential gene which was THBS1. The ORA analysis results indicated that differentially expressed genes, including THBS1, were significantly enriched in the PI3K-Akt signaling pathway.
CONCLUSIONS:Aloe emodin may inhibit the PI3K-Akt pathway by downregulating THBS1, thereby reducing the proliferation activity of fibroblasts derived from postoperative palatal scar tissue.
