miR-302a-3p targeting lysosomal-associated membrane protein 5 inhibits the invasion and metastasis of oral squamous cell carcinoma.
10.7518/hxkq.2025.2024409
- Author:
Li YU
1
;
Tiejun ZHOU
2
;
Xiao WU
3
;
Xinhong LIN
3
;
Xiaoyan ZHANG
1
;
Yongxian LAI
3
;
Xinyue LIAO
3
;
Hang SI
3
;
Yun FENG
3
;
Jie JIAN
4
;
Yan FENG
3
Author Information
1. Dept. of Periodontal and Oral Mucosal Disease, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.
2. Dept. of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
3. Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, China.
4. Deyang Stomatological Hospital, Deyang 618000, China.
- Publication Type:Journal Article
- Keywords:
inva-sion and metastasis;
lysosomal-associated membrane protein 5;
microRNA-302a-3p;
oral squamous cell carcinoma
- MeSH:
MicroRNAs/metabolism*;
Mouth Neoplasms/metabolism*;
Humans;
Animals;
Carcinoma, Squamous Cell/genetics*;
Neoplasm Invasiveness;
Cell Proliferation;
Mice, Nude;
Cell Movement;
Lysosomal Membrane Proteins/genetics*;
Mice;
Cell Line, Tumor;
Neoplasm Metastasis
- From:
West China Journal of Stomatology
2025;43(4):547-558
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:This study aimed to explore the expression of lysosomal-associated membrane protein 5 (LAMP5) and microRNA (miR)-302a-3p in oral squamous cell carcinoma (OSCC) and their functional mechanism on the invasion and metastasis of OSCC.
METHODS:The expression of LAMP5 in OSCC and its sensitivity as a prognostic indicator were analyzed on the basis of The Cancer Genome Atlas database. Western blot, quantitative reverse transcription polymerase chain reaction, and cell immunocytochemistry were used to detect the expression of LAMP5 in OSCC tissues and cells. The effect of LAMP5 on the proliferation, migration, and invasion of OSCC cells was evaluated through cell counting kit-8, immunocytochemistry, migration, and invasion assays, respectively. The miRNA targeting prediction websites were used to predict the miR that regulates LAMP5 and verify the targeted regulatory effect of miR-302a-3p on LAMP5. The effect of LAMP5 knockdown on OSCC tumor growth was evaluated in a nude mouse tumorigenesis model.
RESULTS:LAMP5 was highly expressed in OSCC tissues and cells. It showed high sensitivity in the early diagnosis of OSCC. LAMP5 knockdown significantly inhibited the proliferation, migration, and invasion of OSCC cells, whereas LAMP5 overexpression increased these cell activities. The expression of LAMP5 was regulated by miR-302a-3p. In vivo, LAMP5 knockdown significantly inhibited the growth of OSCC tumor.
CONCLUSIONS:LAMP5 promotes the malignant progression of OSCC by enhancing the proliferation, migration, and invasion of OSCC cells. The expression of LAMP5 is negatively regulated by miR-302a-3p.
