Effects of initial periodontal therapy on the formation of neutrophil extracellular traps in gingival crevicular fluid in patients with severe periodontitis.
10.7518/hxkq.2024.2024162
- Author:
Lanqing FU
1
;
Xinyu HAO
1
;
Wenbo QIAN
1
;
Ying SUN
1
Author Information
1. Dept. of Periodontology, The Affiliated Stomatological Hospital of Nanjing Medical University & State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases & Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China.
- Publication Type:Journal Article
- Keywords:
gingival crevicular fluid;
initial periodontal therapy;
neutrophil extracellular trap;
periodontitis
- MeSH:
Humans;
Gingival Crevicular Fluid;
Extracellular Traps/metabolism*;
Porphyromonas gingivalis/isolation & purification*;
Aggregatibacter actinomycetemcomitans/isolation & purification*;
Periodontitis/metabolism*;
Tumor Necrosis Factor-alpha/analysis*;
Prevotella intermedia/isolation & purification*;
Interleukin-8/analysis*;
Male;
Female;
Middle Aged;
Periodontal Index;
Adult
- From:
West China Journal of Stomatology
2025;43(1):46-52
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:This study aimed to observe the effects of initial periodontal therapy on the level of neutrophil extracellular traps (NETs) in gingival crevicular fluid (GCF) of patients with severe periodontitis and to analyze the factors related to the formation of NETs.
METHODS:Thirty-one patients with stage Ⅲ-Ⅳ periodontitis were recruited. Clinical periodontal parameters, including plaque index (PLI), gingival index (GI), probing depth (PD), and clinical atta-chment loss (CAL), were recorded before and 6-8 weeks after initial periodontal therapy. Levels of NETs in GCF were detected by immunofluorescence staining. Quantities of total bacteria, Porphyromonas gingivalis (P. gingivalis), Aggregatibacter actinomycetemcomitans (A. actionomycetemcomitans) and Prevotella intermedia (P. intermedia)in unattached subgingival plaque were determined by real-time quantitative PCR, and levels of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) in GCF were explored by enzyme-linked immunosorbent assay. In addition, the correlations between the level of NETs and the above indicators were analyzed.
RESULTS:After initial periodontal therapy, the level of NETs in GCF, PLI, GI, PD, and CAL; quantities of total bacteria, P. gingivalis, A. actinomycetemcomitans, and P. itermedia; and levels of IL-8 and TNF-α significantly decreased (P<0.05). We observed strong positive correlations between the level of NETs and PLI, GI, PD, CAL, the amount of total bacteria, P. gingivalis, TNF-α, and IL-8 (P<0.05).
CONCLUSIONS:Initial periodontal therapy might decrease the level of NETs in GCF from patients with severe periodontitis, which might be positively correlated with the quantities of P. gingivalis andthe levels of TNF-α and IL-8 in GCF.
