Serum Lipidomics Profiling to Identify Potential Biomarkers of Ischemic Stroke: A Pilot Study in Chinese Adults.

Ji Jun SHI; Zu Jiao NIE; Shu Yao WANG; Hao ZHANG; Xin Wei LI; Jia Ling YAO; Yi Bing JIN; Xiang Dong YANG; Xue Yang ZHANG; Ming Zhi ZHANG; Hao PENG

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Serum Lipidomics Profiling to Identify Potential Biomarkers of Ischemic Stroke: A Pilot Study in Chinese Adults.

Author: Ji Jun SHI ¹ ; Zu Jiao NIE ² ; Shu Yao WANG ³ ; Hao ZHANG ² ; Xin Wei LI ⁴ ; Jia Ling YAO ² ; Yi Bing JIN ² ; Xiang Dong YANG ² ; Xue Yang ZHANG ² ; Ming Zhi ZHANG ² ; Hao PENG ⁵
Author Information

1. Department of Neurology and Clinical Research Center of Neurological Disease, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China.
2. Department of Epidemiology, School of Public Health, MOE Key Laboratory of Geriatric Diseases and Immunology, Suzhou Medical College of Soochow University, Suzhou 215123, Jiangsu, China.
3. Department of Neurology, Tongliao City Hospital, Tongliao 028000, Inner Mongolia, China.
4. Department of Nursing, School of Nursing, Suzhou Medical College of Soochow University, Suzhou 215123, Jiangsu, China.
5. Department of Epidemiology, School of Public Health, MOE Key Laboratory of Geriatric Diseases and Immunology, Suzhou Medical College of Soochow University, Suzhou 215123, Jiangsu, China;Taixing Second People's Hospital, Suzhou Medical College of Soochow University, Taizhou 225400, Jiangsu, China.
Publication Type:Journal Article
Keywords: Case-control study; Ischemic stroke; Lipidomics; Lipids
MeSH: Humans; Pilot Projects; Lipidomics; Male; Female; Biomarkers/blood*; Middle Aged; Ischemic Stroke/blood*; Aged; China; Lipids/blood*; Adult; Case-Control Studies; East Asian People
From: Biomedical and Environmental Sciences 2025;38(8):918-925
CountryChina
Language:English
Abstract: OBJECTIVE:Lipid oxidation is involved in the pathogenesis of atherosclerosis and may be contribute to the development of Ischemic stroke (IS). However, the lipid profiles associated with IS have been poorly studied. We conducted a pilot study to identify potential IS-related lipid molecules and pathways using lipidomic profiling.
METHODS:Serum lipidomic profiling was performed using LC-MS in 20 patients with IS and 20 age- and sex-matched healthy controls. Univariate and multivariate analyses were simultaneously performed to identify the differential lipids. Multiple testing was controlled for using a false discovery rate (FDR) approach. Enrichment analysis was performed using MetaboAnalyst software.
RESULTS:Based on the 294 lipids assayed, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) models were used to distinguish patients with IS from healthy controls. Fifty-six differential lipids were identified with an FDR-adjusted P less than 0.05 and variable influences in projection (VIP) greater than 1.0. These lipids were significantly enriched in glycerophospholipid metabolism (FDR-adjusted P = 0.009, impact score = 0.216).
CONCLUSIONS:Serum lipid profiles differed significantly between patients with IS and healthy controls. Thus, glycerophospholipid metabolism may be involved in the development of IS. These results provide initial evidence that lipid molecules and their related metabolites may serve as new biomarkers and potential therapeutic targets for IS.