Antibiotic-Depleted Lung Microbiota Modulates Surfactant Proteins Expression and Reduces Experimental Silicosis.
- Author:
Qiang ZHOU
1
;
Mei Yu CHANG
2
;
Ning LI
2
;
Yi GUAN
2
;
San Qiao YAO
1
Author Information
- Publication Type:Journal Article
- Keywords: 16s rRNA sequencing; Antibiotics; Fibrosis; Lung microbiota; Surfactant proteins
- MeSH: Animals; Silicosis/prevention & control*; Lung/metabolism*; Mice; Anti-Bacterial Agents/pharmacology*; Microbiota/drug effects*; Silicon Dioxide/toxicity*; Mice, Inbred C57BL; Male; Pulmonary Surfactant-Associated Proteins/genetics*
- From: Biomedical and Environmental Sciences 2025;38(4):469-483
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:Recent studies have overturned the traditional concept of the lung as a "sterile organ" revealing that pulmonary microbiota dysbiosis and abnormal surfactant proteins (SPs) expression are involved in the progression of silicosis. This study aimed to investigate the relationship between abnormal SPs expression and dysbiosis of lung microbiota in silica-induced lung fibrosis, providing insights into mechanisms of silicosis.
METHODS:Lung pathology, SPs expression, and microbiota composition were evaluated in silica-exposed mice. A mouse model of antibiotic-induced microbiota depletion was established, and alveolar structure and SPs expression were assessed. The roles of the lung microbiota and SPs in silicosis progression were further evaluated in mice with antibiotic-induced microbiota depletion, both with and without silica exposure.
RESULTS:Silica exposure induced lung inflammation and fibrosis, along with increased expression of SP-A expression. Antibiotics (Abx)-induced microbiota depletion elevated SP-A and SP-D expression. Furthermore, silica exposure altered lung microbiota composition, enriching potentially pathogenic taxa. However, antibiotic-induced microbiota depletion prior to silica exposure reduced silica-mediated lung fibrosis and inflammation.
CONCLUSION:Lung microbiota is associated with silica-induced lung injury. Overproduction of SP-A and SP-D, induced by Abx-induced microbiota depletion, may enhance the resistance of mouse lung tissue to silica-induced injury.
