Research Advances in the Construction and Application of Intestinal Organoids.

Qing Xue MENG; Hong Yang YI; Peng WANG; Shan LIU; Wei Quan LIANG; Cui Shan CHI; Chen Yu MAO; Wei Zheng LIANG; Jun XUE; Hong Zhou LU

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Research Advances in the Construction and Application of Intestinal Organoids.

Author: Qing Xue MENG ¹ ; Hong Yang YI ² ; Peng WANG ³ ; Shan LIU ⁴ ; Wei Quan LIANG ⁵ ; Cui Shan CHI ⁶ ; Chen Yu MAO ⁷ ; Wei Zheng LIANG ⁸ ; Jun XUE ⁹ ; Hong Zhou LU ²
Author Information

1. Technology Department, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei, China;Hebei Provincial Key Laboratory of Systems Biology and Gene Regulation, Central Laboratory, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei, China.
2. National Clinical Research Centre for Infectious Diseases, The Third People's Hospital of Shenzhen and The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, Guangdong, China.
3. Hebei North University, Zhangjiakou 075000, Hebei, China.
4. Bioimaging Core of Shenzhen Bay Laboratory, Shenzhen 518132, Guangdong, China.
5. FAW Jiefang, Changchun 130011, Jilin, China.
6. Huigu School of High-tech Industrial Development Zone, Changchun 130012, Jilin, China.
7. University of Pennsylvania, PA 19104, United States.
8. Hebei Provincial Key Laboratory of Systems Biology and Gene Regulation, Central Laboratory, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei, China.
9. Hebei Provincial Key Laboratory of Systems Biology and Gene Regulation, Department of Surgery, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei, China.
Publication Type:Review
Keywords: 3D culture; Colorectal cancer; Disease model; Intestinal organoids; Organ transplantation; Pluripotent stem cells; Regenerative medicine; Tumor organoids
MeSH: Organoids/cytology*; Intestines/physiology*; Humans; Animals; Pluripotent Stem Cells
From: Biomedical and Environmental Sciences 2025;38(2):230-247
CountryChina
Language:English
Abstract: The structure of intestinal tissue is complex. In vitro simulation of intestinal structure and function is important for studying intestinal development and diseases. Recently, organoids have been successfully constructed and they have come to play an important role in biomedical research. Organoids are miniaturized three-dimensional (3D) organs, derived from stem cells, which mimic the structure, cell types, and physiological functions of an organ, making them robust models for biomedical research. Intestinal organoids are 3D micro-organs derived from intestinal stem cells or pluripotent stem cells that can successfully simulate the complex structure and function of the intestine, thereby providing a valuable platform for intestinal development and disease research. In this article, we review the latest progress in the construction and application of intestinal organoids.