miR-34c-3p Inhibits Nasopharyngeal Carcinoma Development via Inhibiting M2 Polarization of Macrophages.
- Author:
Yu Zi JI
1
;
Yu Jie WANG
1
;
Ji Qing MA
1
;
Zhi Hua YIN
1
;
Fei LIU
1
;
Yan Zi ZANG
1
;
Guang Ke WANG
1
;
Yong TAI
1
Author Information
- Publication Type:Journal Article
- Keywords: M2 macrophages; Nasopharyngeal carcinoma (NPC); SLC7A11; miR-34c-3p
- MeSH: MicroRNAs/metabolism*; Nasopharyngeal Carcinoma/genetics*; Humans; Animals; Nasopharyngeal Neoplasms/genetics*; Macrophages/physiology*; Cell Line, Tumor; Mice; Cell Proliferation; Mice, Inbred BALB C; Cell Movement; Male; Gene Expression Regulation, Neoplastic; Mice, Nude; Female
- From: Biomedical and Environmental Sciences 2025;38(2):219-229
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:miR-34c-3p is down-regulated in nasopharyngeal carcinoma (NPC). The biological role of miR-34c-3p in NPC and its underlying mechanisms are unknown and were explored in this study.
METHODS:Flow cytometry and immunohistochemical staining were employed to detect cluster of differentiation 86 (CD86) and cluster of differentiation 206 (CD206) expression; quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were employed to examine mRNA expression and protein levels; cell counting kit-8 (CCK8) and transwell assays were employed to assess cell proliferation, migration, and invasion; and hematoxylin-eosin (HE) staining was employed to assess pathological changes in tumor tissues.
RESULTS:Our results revealed that the miR-34c-3p mimic markedly inhibited M2 polarization of macrophages by targeting SLC7A11, and M2 macrophages transfected with the miR-34c-3p mimic inhibited the proliferation, migration, and invasion of NPC cells. The in vivo experiments further confirmed that miR-34c-3p mimics blocked tumor growth and reduced inflammatory infiltration in tumor tissues.
CONCLUSION:This study provides novel insights into the pathogenesis of NPC and a new treatment strategy.
