bla NDM-1 Carried by a Transferable Plasmid in a Salmonella Strain Isolated from Healthy Individuals.

Wei ZENG; Ming LUO; Pengcheng DU; Zhenpeng LI; Yao PENG; Mengyu WANG; Wenxuan ZHAO; Huayao ZHANG; Yang LI; Pengjie LUO; Yannong WU; Jialiang XU; Xu LI; Xin LU; Biao KAN

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bla _NDM-1 Carried by a Transferable Plasmid in a Salmonella Strain Isolated from Healthy Individuals.

Author: Wei ZENG ¹ ; Ming LUO ² ; Pengcheng DU ³ ; Zhenpeng LI ⁴ ; Yao PENG ⁴ ; Mengyu WANG ⁵ ; Wenxuan ZHAO ⁴ ; Huayao ZHANG ⁴ ; Yang LI ⁴ ; Pengjie LUO ⁶ ; Yannong WU ² ; Jialiang XU ⁷ ; Xu LI ⁷ ; Xin LU ⁴ ; Biao KAN ⁵
Author Information

1. Yantai Center for Disease Control and Prevention, Yantai 264003, Shandong, China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.;School of Public Health, Shandong University, Jinan 250012, Shandong, China.
2. Yulin Center for Disease Control and Prevention, Yulin 537000, Guangxi, China.
3. Qitan Technology Ltd., Chengdu 610044, Sichuan, China.
4. National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China..
5. National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.;School of Public Health, Shandong University, Jinan 250012, Shandong, China.
6. NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100022, China.
7. School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China.
Publication Type:Journal Article
Keywords: Carbapenems; Healthy individual; IncHI2/IncHI2A plasmid; NDM-1; Salmonella Typhimurium
MeSH: beta-Lactamases/genetics*; Plasmids/genetics*; Humans; Anti-Bacterial Agents/pharmacology*; China; Microbial Sensitivity Tests; Salmonella typhimurium/isolation & purification*; Salmonella/isolation & purification*; Salmonella Infections/microbiology*
From: Biomedical and Environmental Sciences 2024;37(11):1252-1261
CountryChina
Language:English
Abstract: OBJECTIVE:Our study aimed to conduct genomic characterization of Salmonella strains carrying the bla _NDM-1 gene in the intestinal tract of healthy individuals. The objectives were to underscore the importance of genomic surveillance for drug resistance in both commensal and pathogenic bacteria among healthy populations, and to establish protocols for regulating drug resistance plasmids based on the completion of a comprehensive map of drug resistance plasmid genomes.
METHODS:We performed antimicrobial susceptibility testing and employed second- and third-generation sequencing techniques to analyze Salmonella strains harboring the bla _NDM-1 gene, to surveil drug-resistant bacteria in the intestines of healthy subjects. Sequence comparison was conducted using both core- and pan-genome approaches. Concurrently, conjugation experiments were carried out to assess the efficiency of plasmid transfer.
RESULTS:We isolated a carbapenem-resistant Salmonella enterica serovar Typhimurium strain from a healthy food worker in China. This strain harbored an IncHI2/IncHI2A plasmid carrying bla _NDM-1 along with multiple antibiotic resistance genes (ARGs). Our findings highlight the potential for asymptomatic carriers to facilitate the transmission of ARGs. Pan-genomic analysis revealed that bla _NDM-1-positive plasmids could traverse bacterial species barriers, facilitating cross-host transmission.
CONCLUSION:This study marks the first detection of bla _NDM-1 in Salmonella strains isolated from healthy individuals. We underscore the risk associated with the transmission of conjugative hybrid plasmids carrying bla _NDM-1, which have the potential to be harbored and transmitted among healthy individuals. Enhanced surveillance of drug-resistant pathogens and plasmids in the intestinal microbiota of healthy individuals could provide insights into the risk of ARG transmission and pathways for population-wide dissemination via ARG transfer factors.