Research progress on new techniques and methods for identifying active ingredients in traditional Chinese medicine.
10.1016/S1875-5364(25)60982-4
- Author:
Jiaxin ZHANG
1
;
Xinhao ZHU
1
;
Chaofeng ZHANG
2
;
Wangning ZHANG
3
,
4
;
Jiangwei TIAN
5
Author Information
1. State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
2. State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address: zhangchaofeng@cpu.edu.cn.
3. State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address: 18435148209@
4. com.
5. State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address: jwtian@cpu.edu.cn.
- Publication Type:Review
- Keywords:
Active ingredients;
High-content screening;
Molecular probe;
New techniques and methods
- MeSH:
Drugs, Chinese Herbal/chemistry*;
Medicine, Chinese Traditional/methods*;
Humans;
Drug Discovery/methods*;
Animals
- From:
Chinese Journal of Natural Medicines (English Ed.)
2025;23(10):1153-1170
- CountryChina
- Language:English
-
Abstract:
Recent years have witnessed significant advances in the development of novel techniques and methodologies for identifying active ingredients in traditional Chinese medicine (TCM), substantially advancing research and development efforts. Spectrum-effect correlation analysis, affinity ultrafiltration, high-content screening (HCS) imaging, and cell membrane chromatography (CMC) have emerged as essential tools, effectively linking TCM chemical constituents to their biological effects, thereby enabling efficient active ingredient screening. Additionally, molecular interaction analysis provides deeper insights into TCM-biomolecule interaction mechanisms, enhancing understanding of its therapeutic potential. Computer-aided techniques facilitate TCM active ingredient identification, optimizing the screening process for efficiency and cost-effectiveness. Molecular probe technology, as an emerging methodology, enables precise and rapid screening for novel therapeutic drug discovery. Ongoing technological advancement in this field indicates promising future developments, potentially leading to more effective and targeted TCM-based therapies.
