TSZAF monomer combination downregulates the Wnt/β-catenin signaling pathway and inhibits neutrophil recruitment to prevent lung cancer metastasis.
10.1016/S1875-5364(25)60973-3
- Author:
Pan YU
1
;
Jialiang YAO
1
;
Long ZHANG
1
;
Yanhong WANG
1
;
Xinyi LU
1
;
Jiajun LIU
2
;
Zujun QUE
2
;
Yao LIU
1
;
Qian BA
3
;
Jiwei LIU
4
;
Yan WU
5
,
6
;
Jianhui TIAN
7
Author Information
1. Clinical Oncology Center, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
2. Institute of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
3. Science and Technology Innovation Center, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
4. Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China.
5. Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. Electronic address: drwuyan@
6. com.
7. Clinical Oncology Center, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China; Institute of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China. Electronic address: tjhhawk@shutcm.edu.cn.
- Publication Type:Journal Article
- Keywords:
CXCL5;
Circulating tumor cells (CTCs);
Lung cancer;
Metastasis;
Neutrophil;
Wnt/β-catenin
- MeSH:
Lung Neoplasms/genetics*;
Wnt Signaling Pathway/drug effects*;
Animals;
Humans;
Drugs, Chinese Herbal/pharmacology*;
Mice;
Neoplasm Metastasis/prevention & control*;
Cell Proliferation/drug effects*;
Cell Line, Tumor;
Neutrophil Infiltration/drug effects*;
Down-Regulation/drug effects*;
Cell Movement/drug effects*;
beta Catenin/genetics*;
Apoptosis/drug effects*;
Mice, Inbred C57BL;
Male;
Neoplastic Cells, Circulating/drug effects*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2025;23(9):1069-1079
- CountryChina
- Language:English
-
Abstract:
Metastasis remains the primary cause of cancer-related mortality worldwide. Circulating tumor cells (CTCs) represent critical targets for metastasis prevention and treatment. Traditional Chinese medicine may prevent lung cancer metastasis through long-term intervention in CTC activity. Tiao-Shen-Zhi-Ai Formular (TSZAF) represents a Chinese medicine compound prescription utilized clinically for lung cancer treatment. This study combined three principal active ingredients from TSZAF into a novel TSZAF monomer combination (TSZAF mc) to investigate its anti-metastatic effects and mechanisms. TSZAF mc demonstrated significant inhibition of proliferation, migration, and invasion in CTC-TJH-01 and LLC cells, while inducing cellular apoptosis in vitro. Moreover, TSZAF mc substantially inhibited LLC cell growth and metastasis in vivo. Mechanistically, TAZSF mc significantly suppressed the Wnt/β-catenin signaling pathway and CXCL5 expression in lung cancer cells and tissues. Additionally, TAZSF mc notably reduced neutrophil infiltration in metastatic lesions. These findings indicate that TSZAF mc inhibits lung cancer growth and metastasis by suppressing the Wnt/β-catenin signaling pathway and reducing CXCL5 secretion, thereby decreasing neutrophil recruitment and infiltration. TSZAF mc demonstrates potential as an effective therapeutic agent for lung cancer metastasis.
