Ten new lignans with anti-inflammatory activities from the leaves of Illicium dunnianum.
10.1016/S1875-5364(25)60934-4
- Author:
Ting LI
1
;
Xiaoqing HE
1
;
Dabo PAN
2
;
Xiaochun ZENG
1
;
Siying ZENG
1
;
Zhenzhong WANG
3
;
Xinsheng YAO
1
;
Wei XIAO
4
,
5
;
Haibo LI
6
;
Yang YU
5
,
7
Author Information
1. Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy; State Key Laboratory of Bioactive Molecules and Druggability Assessment; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China; and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China.
2. Department of Medical Technology, Qiandongnan Vocational & Technical College for Nationalities, Kaili 556000, China.
3. State Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
4. State Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China. Electronic address: xw_kanion@
5. com.
6. State Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China. Electronic address: lihaibo1985124@sina.com.
7. Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy; State Key Laboratory of Bioactive Molecules and Druggability Assessment; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China; and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China. Electronic address: 1018yuyang@
- Publication Type:Journal Article
- Keywords:
Anti-inflammatory activity;
Illicium dunnianum;
Lignan;
Structural elucidation
- MeSH:
Lignans/isolation & purification*;
Plant Leaves/chemistry*;
Anti-Inflammatory Agents/isolation & purification*;
Mice;
Animals;
Molecular Structure;
Plant Extracts/pharmacology*;
Illicium/chemistry*;
Cyclooxygenase 2/immunology*;
Interleukin-6/immunology*;
Nitric Oxide/metabolism*;
Cell Line;
Tumor Necrosis Factor-alpha/immunology*;
Nitric Oxide Synthase Type II/immunology*;
Lipopolysaccharides
- From:
Chinese Journal of Natural Medicines (English Ed.)
2025;23(8):990-996
- CountryChina
- Language:English
-
Abstract:
The anti-inflammatory phytochemical investigation of the leaves of Illicium dunnianum (I. dunnianum) resulted in the isolation of five pairs of new lignans (1-5), and 7 known analogs (6-12). The separation of enantiomer mixtures 1-5 to 1a/1b-5a/5b was achieved using a chiral column with acetonitrile-water mixtures as eluents. The planar structures of 1-2 were previously undescribed, and the chiral separation and absolute configurations of 3-5 were reported for the first time. Their structures were determined through comprehensive spectroscopic data analysis [nuclear magnetic resonance (NMR), high-resolution electrospray ionization mass (HR-ESI-MS), infrared (IR), and ultraviolet (UV)] and quantum chemistry calculations (ECD). The new isolates were evaluated by measuring their inhibitory effect on NO in lipopolysaccharide (LPS)-stimulated BV-2 cells. Compounds 1a, 3a, 3b, and 5a demonstrated partial inhibition of NO production in a concentration-dependent manner. Western blot and real-time polymerase chain reaction (PCR) assays revealed that 1a down-regulated the messenger ribonucleic acid (mRNA) levels of tumor necrosis factor α (TNF-α), interleukin-6 (IL-6), COX-2, and iNOS and the protein expressions of COX-2 and iNOS. This research provides guidance and evidence for the further development and utilization of I. dunnianum.
