Combining label-free quantitative proteomics and 2D-DIGE to identify the potential targets of Sini Decoction acting on myocardial infarction.
10.1016/S1875-5364(25)60937-X
- Author:
Fei FENG
1
;
Weiyue ZHANG
2
;
Yan CAO
3
;
Diya LV
4
;
Yifeng CHAI
5
;
Dandan GUO
6
,
7
;
Xiaofei CHEN
7
,
8
Author Information
1. School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China; Department of Pharmacy, Shanghai Changhai Hospital, Naval Medical University (Second Military Medical University), Shanghai 200433, China.
2. School of Nursing, Beijing University of Chinese Medicine, Beijing 102488, China.
3. School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai 200433, China.
4. School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China.
5. School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China. Electronic address: yfchai@smmu.edu.cn.
6. School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China. Electronic address: dana_guo0826@
7. com.
8. School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China. Electronic address: xfchen2010@
- Publication Type:Journal Article
- Keywords:
Drug affinity response target stability;
Label-free quantitative proteomics;
Myocardial infarction;
Sini Decoction
- MeSH:
Myocardial Infarction/genetics*;
Proteomics/methods*;
Drugs, Chinese Herbal/chemistry*;
Animals;
Protein Disulfide-Isomerases/genetics*;
Male;
Two-Dimensional Difference Gel Electrophoresis/methods*;
Humans;
Rats;
Rats, Sprague-Dawley;
Electrophoresis, Gel, Two-Dimensional
- From:
Chinese Journal of Natural Medicines (English Ed.)
2025;23(8):1016-1024
- CountryChina
- Language:English
-
Abstract:
Sini Decoction (SNT) is a traditional formula recognized for its efficacy in warming the spleen and stomach and dispersing cold. However, elucidating the mechanism of action of SNT remains challenging due to its complex multiple components. This study utilized a synergistic approach combining two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE)-based drug affinity responsive target stability (DARTS) with label-free quantitative proteomics techniques to identify the direct and indirect protein targets of SNT in myocardial infarction. The analysis identified 590 proteins, with 30 proteins showing significant upregulation and 51 proteins showing downregulation when comparing the SNT group with the model group. Through the integration of 2D-DIGE DARTS with proteomics data and pharmacological assessments, the findings indicate that protein disulfide-isomerase A3 (PDIA3) may serve as a potential protein target through which SNT provides protective effects on myocardial cells during myocardial infarction.
