Withanolide derivatives from Physalis angulata var. villosa and their cytotoxic activities.
10.1016/S1875-5364(25)60881-8
- Author:
Peng WANG
1
;
Jue YANG
1
;
Yu ZHANG
1
;
Jun JIN
1
;
Meijun CHEN
1
;
Xiaojiang HAO
2
;
Chunmao YUAN
3
,
4
;
Ping YI
5
Author Information
1. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 561113, China; Natural Products Research Center of Guizhou Province, Guiyang 550014, China.
2. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 561113, China; Natural Products Research Center of Guizhou Province, Guiyang 550014, China; Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
3. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 561113, China; Natural Products Research Center of Guizhou Province, Guiyang 550014, China. Electronic address: yuanchunmao01@
4. com.
5. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 561113, China; Natural Products Research Center of Guizhou Province, Guiyang 550014, China. Electronic address: yiping2100@aliyun.com.
- Publication Type:Journal Article
- Keywords:
Anti-cancer;
Isolation and identification;
Physalis angulata var. villosa;
Withanolide derivatives
- MeSH:
Withanolides/isolation & purification*;
Physalis/chemistry*;
Humans;
Molecular Structure;
Cell Line, Tumor;
Antineoplastic Agents, Phytogenic/isolation & purification*;
Cell Proliferation/drug effects*;
Plant Leaves/chemistry*;
Plant Extracts/pharmacology*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2025;23(6):762-768
- CountryChina
- Language:English
-
Abstract:
A comprehensive phytochemical investigation of the leaves and twigs of Physalis angulata. var. villosa resulted in the isolation of 23 withanolide derivatives, including one novel 13,20-γ-lactone withanolide derivative (1) and three new withanolide derivatives (2-4). Architecturally, physalinin A (1) represents the first identified type B withanolide featuring a 13,20-γ-lactone moiety. The molecular structures of all isolates were elucidated using an integrated approach combining nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), infrared (IR) spectroscopy, and quantum chemical calculations to confirm structural assignments. The antiproliferative activities of all isolated withanolides were evaluated against four human cancer cell lines (HEL, HCT-116, Colo320DM, and MDA-MB-231). Among them, eight derivatives (2, 5-8, 14, 15, and 23) exhibited significant inhibitory effects, with half-maximal inhibitory concentration (IC50) values of 0.18 ± 0.03 to 17.02 ± 0.21 μmol·L-1. Structure-activity relationship (SAR) analysis suggested that the presence of an epoxide ring enhances anticancer activity, potentially through increased reactivity or specific interactions with molecular targets involved in cancer progression. These findings underscore the pharmacological potential of withanolides as promising lead compounds for the development of novel anticancer therapeutics.
