Anti-cancer and anti-inflammatory effects of flavan-4-ol and flavan glycosides from the roots of Pronephrium penangianum.
10.1016/S1875-5364(25)60814-4
- Author:
Feibing HUANG
1
;
Yong YANG
1
;
Qingling XIE
1
;
Hanwen YUAN
1
;
Muhammad AAMER
2
;
Yuqing JIAN
3
,
4
;
Ye ZHANG
5
;
Wei WANG
6
Author Information
1. TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
2. TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
3. TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: cpujyq2010@
4. com.
5. Shenzhen Futian District Maternity & Child Healthcare Hospital, Shenzhen 518000, China.
6. TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: wangwei402@hotmail.com.
- Publication Type:Journal Article
- Keywords:
Anti-cancer;
Anti-inflammatory;
Flavan-4-ol and Flavan glycosides;
Jixueqi;
Pronephrium penangianum;
Tujia ethnomedicine
- MeSH:
Humans;
Plant Roots/chemistry*;
Glycosides/isolation & purification*;
Anti-Inflammatory Agents/isolation & purification*;
Flavonoids/isolation & purification*;
Cell Proliferation/drug effects*;
Antineoplastic Agents, Phytogenic/isolation & purification*;
Molecular Structure;
Apoptosis/drug effects*;
Cell Line, Tumor;
Tumor Necrosis Factor-alpha/immunology*;
Drugs, Chinese Herbal/pharmacology*;
Interleukin-6/immunology*;
Animals;
Mice
- From:
Chinese Journal of Natural Medicines (English Ed.)
2025;23(5):593-603
- CountryChina
- Language:English
-
Abstract:
Five new flavan-4-ol glycosides jixueqiosides A-E (1-5) and two new flavan glycosides jixueqiosides F and G (6 and 7), along with twelve known flavan-4-ol glycosides (8-19), were isolated from the roots of Pronephrium penangianum. Comprehensive spectral analyses, X-ray single-crystal diffraction, and theoretical electronic circular dichroism (ECD) calculations established structures and absolute configurations. A single crystal structure of flavan-4-ol glycoside (14) was reported for the first time, while the characteristic ECD and NMR data for all isolated flavan-4-ol glycosides (1-5 , 8-19) were analyzed, establishing a set of empirical rules. Activity screening of these isolates showed that 8 and 9 could inhibit the proliferation of MDA-MB-231 and MCF-7 cells with IC50 values of 7.93 ? 2.85 ?mol?L-1 and 5.87 ? 1.58 ?mol?L-1 (MDA-MB-231), and 2.21 ? 1.38 ?mol?L-1 and 3.52 ? 1.55 ?mol?L-1 (MCF-7), respectively. Western blotting and flow cytometry analyses demonstrated that 8 and 9 dose-dependently induced apoptosis in MDA-MB-231 cells by up-regulating BAX, activating caspase-3 and down-regulating BCL-2. Additionally, compound 8 affected autophagy-related proteins, increasing the ratio of LC3-II/LC3-I and Beclin-1 levels to inhibit MDA-MB-231 cell proliferation. Moreover, anti-inflammatory studies indicated that 2, 3, 7, 13, 14, and 18 moderately inhibited tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), and nitric oxide (NO) release.
