Neuroprotective and antidiabetic lanostane-type triterpenoids from the fruiting bodies of Ganoderma theaecolum.
10.1016/S1875-5364(25)60828-4
- Author:
Jiaocen GUO
1
;
Li YANG
2
;
Luting DAI
2
;
Qingyun MA
2
;
Jiaoyang YAN
3
;
Qingyi XIE
2
;
Yougen WU
4
,
5
;
Haofu DAI
6
;
Youxing ZHAO
7
Author Information
1. School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, China; Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
2. Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
3. School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, China.
4. School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, China. Electronic address: wygeng2003@
5. com.
6. Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. Electronic address: daihaofu@itbb.org.cn.
7. Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. Electronic address: zhaoyouxing@itbb.org.cn.
- Publication Type:Journal Article
- Keywords:
Ganoderma theaecolum;
Lanostane nortriterpenoids;
Neuroprotective effects;
PTP1B;
α-Glucosidase
- MeSH:
Humans;
Fruiting Bodies, Fungal/chemistry*;
Triterpenes/isolation & purification*;
Neuroprotective Agents/isolation & purification*;
Protein Tyrosine Phosphatase, Non-Receptor Type 1/metabolism*;
Ganoderma/chemistry*;
Apoptosis/drug effects*;
Hypoglycemic Agents/isolation & purification*;
Molecular Structure;
alpha-Glucosidases/metabolism*;
Cell Line, Tumor;
Reactive Oxygen Species/metabolism*;
Oxidative Stress/drug effects*;
Hydrogen Peroxide/toxicity*;
Molecular Docking Simulation
- From:
Chinese Journal of Natural Medicines (English Ed.)
2025;23(2):245-256
- CountryChina
- Language:English
-
Abstract:
Eight previously undescribed lanostane triterpenoids, including five nortriterpenoids with 26 carbons, ganothenoids A-E (1-5), and three lanostanoids, ganothenoids F-H (6-8), along with 24 known ones (9-32), were isolated from the fruiting bodies of Ganodrma theaecolum. The structures of the novel compounds were elucidated using comprehensive spectroscopic methods, including electronic circular dichroism (ECD) and nuclear magnetic resonance (NMR) calculations. Compounds 1-32 were assessed for their neuroprotective effects against H2O2-induced damage in human neuroblastoma SH-SY5Y cells, as well as their inhibitory activities against protein tyrosine phosphatase 1B (PTP1B) and α-glucosidase. Compound 4 demonstrated the most potent neuroprotective activity against H2O2-induced oxidative stress by suppressing G0/G1 phase cell cycle arrest, reducing reactive oxygen species (ROS) levels, and inhibiting cell apoptosis through modulation of B-cell lymphoma 2 protein (Bcl-2) and Bcl-2 associated X-protein (Bax) protein expression. Compounds 26, 12, and 28 exhibited PTP1B inhibitory activities with IC50 values ranging from 13.92 to 56.94 μmol·L-1, while compound 12 alone displayed significant inhibitory effects on α-glucosidase with an IC50 value of 43.56 μmol·L-1. Additionally, enzyme kinetic analyses and molecular docking simulations were conducted for compounds 26 and 12 with PTP1B and α-glucosidase, respectively.
