Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis.
10.1016/j.jpha.2024.101085
- Author:
Xueqin HUANG
1
;
Yingqi YANG
1
;
Hanlin ZHOU
1
;
Liping HU
1
;
Annan YANG
1
;
Hua JIN
2
;
Biying ZHENG
2
;
Jiang PI
2
;
Jun XU
1
;
Pinghua SUN
1
;
Huai-Hong CAI
3
;
Xujing LIANG
4
;
Bin PAN
4
;
Junxia ZHENG
5
;
Haibo ZHOU
1
Author Information
1. College of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, China.
2. Research Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, School of Medical Technology, Guangdong Medical University, Dongguan, Guangdong, 523000, China.
3. College of Chemistry and Materials Science, Jinan University, Guangzhou, 510632, China.
4. The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, 510632, China.
5. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, China.
- Publication Type:Journal Article
- Keywords:
Au@AgPt nanoarrays;
Cascade response strategy;
Colorimetry;
MNase;
Surface-enhanced Raman scattering
- From:
Journal of Pharmaceutical Analysis
2025;15(2):101085-101085
- CountryChina
- Language:English
-
Abstract:
Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections. Herein, we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering (SERS) and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease (MNase) in serum samples. The trimetallic Au@AgPt nanozymes can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) molecules to SERS-enhanced oxidized TMB (oxTMB), accompanied by the color change from colorless to blue. In the presence of S. aureus, the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads (MBs) to release alkaline phosphatase (ALP), which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away. Using this "on-to-off" triggering strategy, the target S. aureus can be recorded in a wide linear range with a limit of detection of 38 CFU/mL in the colorimetric mode and 6 CFU/mL in the SERS mode. Meanwhile, the MNase-mediated strategy characterized by high specificity and sensitivity successfully discriminated between patients with sepsis (n = 7) and healthy participants (n = 3), as well as monitored the prognostic progression of the disease (n = 2). Overall, benefiting from highly active and dense "hot spot" substrate, MNase-mediated cascade response strategy, and colorimetric/SERS dual-signal output, this methodology will offer a promising avenue for the early diagnosis of S. aureus infection.
