A review on the screening methods for the discovery of natural antimicrobial peptides.
10.1016/j.jpha.2024.101046
- Author:
Bin YANG
1
;
Hongyan YANG
1
;
Jianlong LIANG
1
;
Jiarou CHEN
1
;
Chunhua WANG
1
;
Yuanyuan WANG
1
;
Jincai WANG
2
;
Wenhui LUO
3
;
Tao DENG
1
;
Jialiang GUO
1
Author Information
1. School of Medicine, Foshan University, Foshan, Guangdong, 528000, China.
2. College of Pharmacy, Jinan University, Guangzhou, 510632, China.
3. Guangdong Yifang Pharmaceutical Co., Ltd., Foshan, Guangdong, 528244, China.
- Publication Type:Review
- Keywords:
Antimicrobial peptides;
High-throughput screening;
Mechanism;
Natural products
- From:
Journal of Pharmaceutical Analysis
2025;15(1):101046-101046
- CountryChina
- Language:English
-
Abstract:
Natural antimicrobial peptides (AMPs) are promising candidates for the development of a new generation of antimicrobials to combat antibiotic-resistant pathogens. They have found extensive applications in the fields of medicine, food, and agriculture. However, efficiently screening AMPs from natural sources poses several challenges, including low efficiency and high antibiotic resistance. This review focuses on the action mechanisms of AMPs, both through membrane and non-membrane routes. We thoroughly examine various highly efficient AMP screening methods, including whole-bacterial adsorption binding, cell membrane chromatography (CMC), phospholipid membrane chromatography binding, membrane-mediated capillary electrophoresis (CE), colorimetric assays, thin layer chromatography (TLC), fluorescence-based screening, genetic sequencing-based analysis, computational mining of AMP databases, and virtual screening methods. Additionally, we discuss potential developmental applications for enhancing the efficiency of AMP discovery. This review provides a comprehensive framework for identifying AMPs within complex natural product systems.
