Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement.
10.1016/j.jpha.2023.12.012
- Author:
Zhiqiang ZHANG
1
;
Li WANG
2
;
Xia MA
3
;
Hui WANG
1
Author Information
1. College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450045, China.
2. Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou, 450046, China.
3. College of Animal Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
- Publication Type:Journal Article
- Keywords:
LNT-PBAE-G-ND@OVA;
Molecular mechanisms;
cGAS-STING signaling
- From:
Journal of Pharmaceutical Analysis
2024;14(12):100922-100922
- CountryChina
- Language:English
-
Abstract:
A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid (PBAE-G) polymers. In this study, a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin (LNT-PBAE-G-ND@OVA). The impact of this drug delivery system on the activation and maturation of macrophages was then assessed. Furthermore, LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo. Moreover, treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway, and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules. LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS)-stimulator of interferon genes (STING) signaling plays an integral role.
