TRIM4 modulates the ubiquitin-mediated degradation of hnRNPDL and weakens sensitivity to CDK4/6 inhibitor in ovarian cancer.
10.1007/s11684-024-1103-5
- Author:
Xiaoxia CHE
1
;
Xin GUAN
1
;
Yiyin RUAN
1
;
Lifei SHEN
1
;
Yuhong SHEN
1
;
Hua LIU
1
;
Chongying ZHU
1
;
Tianyu ZHOU
1
;
Yiwei WANG
2
;
Weiwei FENG
3
Author Information
1. Department of Gynecology and Obstetrics, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
2. Department of Gynecology and Obstetrics, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. wyw8Y743@rjh.com.cn.
3. Department of Gynecology and Obstetrics, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. fww12066@rjh.com.cn.
- Publication Type:Journal Article
- Keywords:
CDK4/6 inhibitor;
TRIM4;
hnRNPDL;
ovarian cancer
- MeSH:
Humans;
Female;
Ovarian Neoplasms/pathology*;
Animals;
Tripartite Motif Proteins/genetics*;
Mice;
Cyclin-Dependent Kinase 4/antagonists & inhibitors*;
Cell Line, Tumor;
Cyclin-Dependent Kinase 6/antagonists & inhibitors*;
Protein Kinase Inhibitors/pharmacology*;
Ubiquitin/metabolism*;
Xenograft Model Antitumor Assays;
Ubiquitination;
Antineoplastic Agents/pharmacology*
- From:
Frontiers of Medicine
2025;19(1):121-133
- CountryChina
- Language:English
-
Abstract:
Ovarian cancer is the most lethal malignancy affecting the female reproductive system. Pharmacological inhibitors targeting CDK4/6 have demonstrated promising efficacy across various cancer types. However, their clinical benefits in ovarian cancer patients fall short of expectations, with only a subset of patients experiencing these advantageous effects. This study aims to provide further clinical and biological evidence for antineoplastic effects of a CDK4/6 inhibitor (TQB4616) in ovarian cancer and explore underlying mechanisms involved. Patient-derived ovarian cancer organoid models were established to evaluate the effectiveness of TQB3616. Potential key genes related to TQB3616 sensitivity were identified through RNA-seq analysis, and TRIM4 was selected as a candidate gene for further investigation. Subsequently, co-immunoprecipitation and GST pull-down assays confirmed that TRIM4 binds to hnRNPDL and promotes its ubiquitination through RING and B-box domains. RIP assay demonstrated that hnRNPDL binded to CDKN2C isoform 2 and suppressed its expression by alternative splicing. Finally, in vivo studies confirmed that the addition of siTRIM4 significantly improved the effectiveness of TQB3616. Overall, our findings suggest that TRIM4 modulates ubiquitin-mediated degradation of hnRNPDL and weakens sensitivity to CDK4/6 inhibitors in ovarian cancer treatment. TRIM4 may serve as a valuable biomarker for predicting sensitivity to CDK4/6 inhibitors in ovarian cancer.
