MYB polymorphism molecular marker: A novel molecular marker for authenticity and geographical origin identification of Citri Reticulatae Pericarpium.
10.1016/j.chmed.2025.02.006
- Author:
Qiqing CHENG
1
;
Ziyu TANG
2
;
Yue OUYANG
2
;
Chunsong CHENG
3
;
Chichou LAO
2
;
Hao CUI
4
;
Hua ZHOU
5
;
Yongshu LIANG
6
Author Information
1. School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China.
2. State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Macao 999078, China.
3. Jiangxi Key Laboratory for Sustainable Utilization of Chinese Material Medical Resources, Lushan Botanical Garden, Chinese Academy of Science, Jiujiang 332900, China.
4. Key Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, School of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
5. Chinese Medicine Guangdong Laboratory (Hengqin Laboratory), Guangdong-Macao In-Depth Cooperation Zone in Hengqin, Zhuhai 519031, China.
6. Guangdong Food and Drug Vocational College, Guangzhou 510520, China.
- Publication Type:Journal Article
- Keywords:
Citri Reticulatae Pericarpium;
MYB transcription factor;
genetic diversity;
geographical origin identification;
molecular maker
- From:
Chinese Herbal Medicines
2025;17(2):296-306
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:Citri Reticulatae Pericarpium (Chenpi, CRP) is one of the most used traditional Chinese medicines with great medicinal, dietary and collection values, among which the Citrus reticulata cv. 'Chachi' (Citrus reticulata cv. Chachiensis) from Guangdong Xinhui is the geoherb of CRP. Xinhui CRP in the market was often counterfeited with other varieties or origins, molecular identification method can effectively distinguish different CRP varieties, but it's still a difficult problem to identify the same CRP variety from different origin. It is necessary to discover a new molecular marker to ensure the safe and effective application of Xinhui CRP.
METHODS:We selected one of the most studied transcription factor families in Citrus genus, MYB, to design the specific candidate primers on the conserved region. The primers with good band repeatability and high polymorphism were screened for PCR amplification of the test materials, and the genetic similarity coefficient among different families, genera, species, and origins were calculated. The cluster analysis was performed by unweighted pair group method using arithmetic average (UPGMA).
RESULTS:A total of ten MYB primers were screened out to identify Xinhui CRP from plants from different family (Panax ginseng and Morus alba), genus (Clausena lansium and Zanthoxylum schinifolium), and species (Citrus reticulata, C. sinensis and C. maxima). Furthermore, two from the ten primers, M1 and M10, were found to distinguish Xinhui CRP from other origins. There were 169, 113, 133 and 134 polymorphic bands in the identification of different families, genera, species, and origins respectively, and the accordingly polymorphism ration were 79.88%, 76.87%, 79.20% and 82.84%. Moreover, M1 was discovered to be the best primer to identify Xinhui CRP from other seven origins, the cluster analysis results based on the genetic similarity coefficients were consistent with the geographical distribution.
CONCLUSION:This study established a novel molecular identification method according to MYB transcription factor, which can analyze the potential parental relationship of CRP germplasm, as well as identify the quality and origins of Xinhui CPR.
