Ameliorating vascular endothelial injury for lipolysacharide-induced via mitochondrial targeting function of octaarginine-modified essential oil from Fructus <i>Alpiniae zerumbet</i> (EOFAZ) lipid microspheres.

Lingyan LI; Zengqiu YANG; Qiqi LI; Qianqian GUO; Xingjie WU; Yu'e WANG; Xiangchun SHEN; Ying CHEN; Ling TAO

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Ameliorating vascular endothelial injury for lipolysacharide-induced via mitochondrial targeting function of octaarginine-modified essential oil from Fructus Alpiniae zerumbet (EOFAZ) lipid microspheres.

Author: Lingyan LI ¹ ; Zengqiu YANG ¹ ; Qiqi LI ¹ ; Qianqian GUO ¹ ; Xingjie WU ¹ ; Yu'e WANG ¹ ; Xiangchun SHEN ¹ ; Ying CHEN ¹ ; Ling TAO ¹
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1. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 561113, China.
Publication Type:Journal Article
Keywords: essential oil from Fructus Alpinia zerumbet [fruit of Alpinia zerumbet (Pers.) Burtt.et Smith]; inflammation; lipid microspheres; mitochondrial dysfunction; octaarginine; vascular endothelial injury
From: Chinese Herbal Medicines 2025;17(2):340-351
CountryChina
Language:English
Abstract: OBJECTIVE:To investigate the therapeutic potential of octaarginine (R8)-modified essential oil from Fructus Alpiniae zerumbet (EOFAZ) lipid microspheres (EOFAZ@^R8LM) for cardiovascular therapy.
METHODS:EOFAZ@^R8LM was developed by leveraging the volatilization of EOFAZ and integrating it with the oil phase of LM, followed by surface modification with cell-penetrating peptide R8 to target the site of vascular endothelial injury. The therapeutic effects of this formulation in alleviating lipopolysaccharide-induced vascular endothelial inflammation were evaluated by assessing mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) levels, as well as inflammatory factors interleukin-6 (IL-6) and interleukin-1β (IL-1β) levels.
RESULTS:EOFAZ@^R8LM effectively delivered EOFAZ to the site of injury and specifically targeted the mitochondria in vascular endothelial cells, thereby ameliorating mitochondrial dysfunction through regulation of MMP and reduction of intracellular ROS levels. Moreover, it attenuated the expression levels of IL-6 and IL-1β, exerting protective effects on the vascular endothelium.
CONCLUSION:Our findings highlight the significant therapeutic potential of EOFAZ@^R8LM in cardiovascular therapy, providing valuable insights for developing novel dosage forms utilizing EOFAZ for effective treatment against cardiovascular diseases.