Two new polyketides from Rhodiola tibetica endophytic fungus Penicillium sp. HJT-A-6.
10.1016/j.chmed.2024.06.004
- Author:
Dongliang XIAO
1
;
Xiaobao LI
1
;
Xuemei ZHANG
1
;
Nan JIANG
1
;
Dunzhu LUOSANG
2
;
Weixing FENG
1
;
Xuan LU
1
;
Baomin FENG
1
Author Information
1. College of Life and Health, Dalian University, Dalian 116622, China.
2. Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Tibet 850000, China.
- Publication Type:Journal Article
- Keywords:
Penicillium sp.;
Rhodiola tibetica (HK. f. et Thoms.) S.H. Fu;
polyketi;
seed-germination-promoting activity;
structural characterization
- From:
Chinese Herbal Medicines
2025;17(2):404-408
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To study bioactive compounds from the endophytic fungus Penicillium sp. HJT-A-6 isolated from stem of Rhodiola tibetica, and evaluate its allelopathic activity.
METHODS:The chemical constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were elucidated by extensive spectroscopic analysis and electronic circular dichroism (ECD) calculations. In addition, the allelopathic activity of compound 1 was evaluated by measuring the seed germination rate of R. tibetica.
RESULTS:Two new polyketides 4-hydroxy-3,6-dimethyl-2H-pyran-2-one (1) and penilactone E (2), together with six known compounds walterolactone A (3), 5-hydroxyhexan-4-olide (4), 3-methyl-2-penten-5-olide (5), chaetoquadrin F (6), (Z)-6-acetyl-3-(1,2-dihydroxypropylidene)-5-hydroxy-8-methylchroman-2-one (7) and 4-hydroxy-3-(4-hydroxyhexanoyl)-5-methylfuran-2(5H)-one (8) were isolated from Penicillium sp. HJT-A-6. Compound 1 showed moderate seed-germination-promoting activity at a concentration of 0.001 mg/mL while inhibiting the seed germination at concentrations of 0.1 and 0.01 mg/mL. Compared with the positive drug 6-benzyladenine (6-BA), compound 1 could extend the seed-germination period of R. tibetica (up to 11 d).
CONCLUSION:Two new compounds were isolated from R. tibetica endophytic fungus Penicillium sp. HJT-A-6. Compound 1 displayed plant hormone-like activity, which inhibited the seed germination of the host plant at high concentrations and promoted the seed germination of the host plant at low concentrations. The results not only enrich the chemical constituents of the endophytic fungi isolated from Rhodiola tibetica, but also provide a theoretical basis for understanding the interaction mechanism between Rhodiola tibetica endophytic fungi and the host plant.
