Prespecified dental mesenchymal cells for the making of a tooth.
10.1038/s41368-025-00391-7
- Author:
Eun-Jung KIM
1
;
Hyun-Yi KIM
2
;
Suyeon LEE
1
;
Junsu KIM
1
;
Shujin LI
1
;
Anish Ashok ADPAIKAR
1
;
Thantrira PORNTAVEETUS
3
;
Senthil Kumar BASKARAN
1
;
Jong-Min LEE
1
;
Han-Sung JUNG
4
Author Information
1. Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Korea.
2. NGeneS Inc., Ansan-si, Korea.
3. Center of Excellence in Precision Medicine and Digital Health, Geriatric Dentistry and Special Patients Care, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
4. Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Korea. hsjung@yuhs.ac.
- Publication Type:Journal Article
- MeSH:
Animals;
Mice;
Odontogenesis/genetics*;
Tooth Germ/cytology*;
Mesoderm/cytology*;
Cell Differentiation;
Mesenchymal Stem Cells;
Tooth/embryology*
- From:
International Journal of Oral Science
2025;17(1):67-67
- CountryChina
- Language:English
-
Abstract:
Positional information plays a crucial role in embryonic pattern formation, yet its role in tooth development remains unexplored. In this study, we investigated the regional specification of lingual and buccal dental mesenchyme during tooth development. Tooth germs at the cap stage were dissected from mouse mandibles, and their lingual and buccal mesenchymal regions were separated for bulk RNA sequencing. Gene ontology analysis revealed that odontogenesis, pattern specification, and proliferation-related genes were enriched in the lingual mesenchyme, whereas stem cell development, mesenchymal differentiation, neural crest differentiation, and regeneration-related genes were predominant in the buccal mesenchyme. Reaggregation experiments using Wnt1creERT/+; R26RtdT/+ and WT mouse models demonstrated that lingual mesenchyme contributes to tooth formation, while buccal mesenchyme primarily supports surrounding tissues. Furthermore, only the lingual part of tooth germs exhibited odontogenic potential when cultured in vitro and transplanted under the kidney capsule. Bulk RNA transcriptomic analysis further validated the regional specification of the lingual and buccal mesenchyme. These findings provide novel insights into the molecular basis of positional information in tooth development and pattern formation.
