Single-cell transcriptomics identifies PDGFRA+ progenitors orchestrating angiogenesis and periodontal tissue regeneration.
10.1038/s41368-025-00384-6
- Author:
Jianing LIU
1
;
Junxi HE
1
;
Ziqi ZHANG
1
;
Lu LIU
1
;
Yuan CAO
1
;
Xiaohui ZHANG
1
;
Xinyue CAI
1
;
Xinyan LUO
1
;
Xiao LEI
1
;
Nan ZHANG
1
;
Hao WANG
1
;
Ji CHEN
2
;
Peisheng LIU
1
;
Jiongyi TIAN
1
;
Jiexi LIU
1
;
Yuru GAO
1
;
Haokun XU
1
;
Chao MA
1
;
Shengfeng BAI
1
;
Yubohan ZHANG
1
;
Yan JIN
1
;
Chenxi ZHENG
3
;
Bingdong SUI
4
;
Fang JIN
5
Author Information
1. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
2. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Oral Implantology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
3. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, China. chenxizheng@fmmu.edu.cn.
4. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, China. bingdong@fmmu.edu.cn.
5. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China. jinfang@fmmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Receptor, Platelet-Derived Growth Factor alpha/metabolism*;
Humans;
Neovascularization, Physiologic/physiology*;
Dental Sac/cytology*;
Single-Cell Analysis;
Transcriptome;
Mesenchymal Stem Cells/metabolism*;
Bone Regeneration;
Animals;
Dental Papilla/cytology*;
Periodontium/physiology*;
Stem Cells/metabolism*;
Regeneration;
Angiogenesis
- From:
International Journal of Oral Science
2025;17(1):56-56
- CountryChina
- Language:English
-
Abstract:
Periodontal bone defects, primarily caused by periodontitis, are highly prevalent in clinical settings and manifest as bone fenestration, dehiscence, or attachment loss, presenting a significant challenge to oral health. In regenerative medicine, harnessing developmental principles for tissue repair offers promising therapeutic potential. Of particular interest is the condensation of progenitor cells, an essential event in organogenesis that has inspired clinically effective cell aggregation approaches in dental regeneration. However, the precise cellular coordination mechanisms during condensation and regeneration remain elusive. Here, taking the tooth as a model organ, we employed single-cell RNA sequencing to dissect the cellular composition and heterogeneity of human dental follicle and dental papilla, revealing a distinct Platelet-derived growth factor receptor alpha (PDGFRA) mesenchymal stem/stromal cell (MSC) population with remarkable odontogenic potential. Interestingly, a reciprocal paracrine interaction between PDGFRA+ dental follicle stem cells (DFSCs) and CD31+ Endomucin+ endothelial cells (ECs) was mediated by Vascular endothelial growth factor A (VEGFA) and Platelet-derived growth factor subunit BB (PDGFBB). This crosstalk not only maintains the functionality of PDGFRA+ DFSCs but also drives specialized angiogenesis. In vivo periodontal bone regeneration experiments further reveal that communication between PDGFRA+ DFSC aggregates and recipient ECs is essential for effective angiogenic-osteogenic coupling and rapid tissue repair. Collectively, our results unravel the importance of MSC-EC crosstalk mediated by the VEGFA and PDGFBB-PDGFRA reciprocal signaling in orchestrating angiogenesis and osteogenesis. These findings not only establish a framework for deciphering and promoting periodontal bone regeneration in potential clinical applications but also offer insights for future therapeutic strategies in dental or broader regenerative medicine.
