Profiling and functional characterization of long noncoding RNAs during human tooth development.
10.1038/s41368-025-00375-7
- Author:
Xiuge GU
1
;
Wei WEI
1
;
Chuan WU
1
;
Jing SUN
1
;
Xiaoshan WU
1
;
Zongshan SHEN
1
;
Hanzhang ZHOU
2
;
Chunmei ZHANG
1
;
Jinsong WANG
1
;
Lei HU
1
;
Suwen CHEN
3
;
Yuanyuan ZHANG
3
;
Songlin WANG
4
;
Ran ZHANG
5
Author Information
1. Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Capital Medical University, Beijing, China.
2. Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.
3. Department of Reproductive Regulation, Beijing Obstetrics and Gynecology Hospital, Beijing Maternal and Child Health Care Hospital, Capital Medical University, Beijing, China.
4. Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Capital Medical University, Beijing, China. slwang@ccmu.edu.cn.
5. Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Capital Medical University, Beijing, China. rani@bjmu.edu.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Humans;
RNA, Long Noncoding/metabolism*;
Odontogenesis/genetics*;
Tooth Germ/embryology*;
Cell Differentiation;
Gene Expression Regulation, Developmental;
Mesoderm/metabolism*;
Tooth/embryology*;
Gene Expression Profiling;
Sequence Analysis, RNA;
Dental Pulp/cytology*
- From:
International Journal of Oral Science
2025;17(1):38-38
- CountryChina
- Language:English
-
Abstract:
The regulatory processes in developmental biology research are significantly influenced by long non-coding RNAs (lncRNAs). However, the dynamics of lncRNA expression during human tooth development remain poorly understood. In this research, we examined the lncRNAs present in the dental epithelium (DE) and dental mesenchyme (DM) at the late bud, cap, and early bell stages of human fetal tooth development through bulk RNA sequencing. Developmental regulators co-expressed with neighboring lncRNAs were significantly enriched in odontogenesis. Specific lncRNAs expressed in the DE and DM, such as PANCR, MIR205HG, DLX6-AS1, and DNM3OS, were identified through a combination of bulk RNA sequencing and single-cell analysis. Further subcluster analysis revealed lncRNAs specifically expressed in important regions of the tooth germ, such as the inner enamel epithelium and coronal dental papilla (CDP). Functionally, we demonstrated that CDP-specific DLX6-AS1 enhanced odontoblastic differentiation in human tooth germ mesenchymal cells and dental pulp stem cells. These findings suggest that lncRNAs could serve as valuable cell markers for tooth development and potential therapeutic targets for tooth regeneration.
