Salvianolic Acid B and Ginsenoside Rg1 Combination Attenuates Cerebral Edema Accompanying Glymphatic Modulation.
10.1007/s12264-025-01490-5
- Author:
Lingxiao ZHANG
1
;
Yanan SHAO
2
;
Zhao FANG
2
;
Siqi CHEN
3
;
Yixuan WANG
2
;
Han SHA
3
;
Yuhan ZHANG
3
;
Linlin WANG
2
;
Yi JIN
4
;
Hao CHEN
5
;
Baohong JIANG
6
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.
2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
3. School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
4. Shenzhen Institute for Drug Control (Shenzhen Testing Center of Medical Devices), Shenzhen, 518057, China.
5. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. haoc@simm.ac.cn.
6. School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China. jiangbh@simm.ac.cn.
- Publication Type:Journal Article
- Keywords:
AQP4;
Cerebral edema;
Glymphatic system;
Ischemic stroke;
MMP9;
SalB/Rg1
- MeSH:
Animals;
Ginsenosides/administration & dosage*;
Brain Edema/etiology*;
Male;
Benzofurans/administration & dosage*;
Glymphatic System/diagnostic imaging*;
Mice;
Infarction, Middle Cerebral Artery/drug therapy*;
Aquaporin 4/metabolism*;
Disease Models, Animal;
Mice, Inbred C57BL;
Matrix Metalloproteinase 9/metabolism*;
Neuroprotective Agents/pharmacology*;
Depsides
- From:
Neuroscience Bulletin
2025;41(11):1909-1923
- CountryChina
- Language:English
-
Abstract:
Cerebral edema is characterized by fluid accumulation, and the glymphatic system (GS) plays a pivotal role in regulating fluid transport. Using the Tenecteplase system, magnesium salt of salvianolic acid B/ginsenoside Rg1 (SalB/Rg1) was injected intravenously into mice 4.5 h after middle cerebral artery occlusion and once every 24 h for the following 72 h. GS function was assessed by Evans blue imaging, near-infrared fluorescence region II (NIR-II) imaging, and magnetic resonance imaging (MRI). SalB/Rg1 had significant effects on reducing the infarct volume and hemorrhagic transformation score, improving neurobehavioral function, and protecting tissue structure, especially inhibiting cerebral edema. Meanwhile, the influx/efflux drainage of GS was enhanced by SalB/Rg1 according to NIR-II imaging and MRI. SalB/Rg1 inhibited matrix metalloproteinase-9 (MMP-9) activity, reduced cleaved β-dystroglycan (β-DG), and stabilized aquaporin-4 (AQP4) polarity, which was verified by colocalization with CD31. Our findings indicated that SalB/Rg1 treatment enhances GS function and attenuates cerebral edema, accompanying the regulation of the MMP9/β-DG/AQP4 pathway.
