Comparative Analysis of Histological Tools for Myelin.
10.1007/s12264-025-01468-3
- Author:
Zihui AN
1
;
Shuo YAN
2
;
Jiayi WANG
1
;
Hanqing QIU
1
;
Binghua XIE
1
;
Shumei FENG
3
;
Mengsheng QIU
1
;
Zhou TAN
4
Author Information
1. Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, 311121, China.
2. PLA Naval Medical Center, Shanghai, 200052, China.
3. Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, 830017, China.
4. Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, 311121, China. tanzhou@hznu.edu.cn.
- Publication Type:Comparative Study
- Keywords:
Corpus callosum;
Demyelination;
Histology;
Myelin;
Spinal cord;
White matter
- MeSH:
Myelin Sheath/metabolism*;
Animals;
Humans;
Demyelinating Diseases/pathology*;
Staining and Labeling/methods*
- From:
Neuroscience Bulletin
2025;41(9):1656-1668
- CountryChina
- Language:English
-
Abstract:
Myelin is an essential structure that facilitates rapid saltatory conduction in the nervous system. Discrepancies in myelin microstructure are a hallmark of numerous neurological disorders, rendering the assessment of myelin integrity and content an indispensable tool in clinical diagnostics and neuroscience research. Extensive research has been dedicated to scrutinizing its biochemical makeup and morphology under normal, pathological, and experimental conditions over the years. In this review, we present an updated summary of the myelin sheath's structure, composition, and developmental trajectory. We systematically enumerate and contrast eight prevalent myelin staining techniques across dimensions of sensitivity, specificity, and resolution, delving into their underlying staining principles. With an initial application of myelin histology on the mouse demyelination model, our review accentuates the accurate delineation of myelination and the microstructural analysis of the myelin sheath. Such insights are anticipated to significantly contribute to the evaluation and understanding of white matter pathologies.
