In Vitro and Animal Studies of Human Natural Killer Cell-Derived Exosomes for the Treatment of Otitis Media.
10.1007/s12264-025-01423-2
- Author:
Zirui ZHAO
1
;
Liqin WANG
1
;
Zhen GUO
2
;
Kanglun JIANG
3
;
Jianghong XU
1
;
Yilai SHU
1
;
Christina Y XU
4
;
Jianning ZHANG
5
;
Yunfeng WANG
6
;
Geng-Lin LI
7
Author Information
1. Department of Otorhinolaryngology, ENT Institute, and NHC Key Laboratory of Hearing Medicine, Eye and ENT Hospital, Fudan University, Shanghai, 200031, China.
2. School of Life Sciences, Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei, 230601, China.
3. Department of Otorhinolaryngology-Head and Neck Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
4. College of Letters and Sciences, University of California Berkeley, Berkeley, CA, 94720, USA.
5. Department of Otolaryngology, Yueyang Hospital of Integrative Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China. eternityz@sina.com.
6. Department of Otorhinolaryngology, ENT Institute, and NHC Key Laboratory of Hearing Medicine, Eye and ENT Hospital, Fudan University, Shanghai, 200031, China. yunfengwang@fudan.edu.cn.
7. Department of Otorhinolaryngology, ENT Institute, and NHC Key Laboratory of Hearing Medicine, Eye and ENT Hospital, Fudan University, Shanghai, 200031, China. genglin.li@fdeent.org.
- Publication Type:Journal Article
- Keywords:
Anti-bacteria;
Extracellular vesicle;
Natural killer cell;
Otitis media
- MeSH:
Killer Cells, Natural/metabolism*;
Exosomes/metabolism*;
Animals;
Humans;
Otitis Media/therapy*;
Staphylococcus aureus/drug effects*;
Disease Models, Animal;
Anti-Bacterial Agents/pharmacology*;
Levofloxacin/pharmacology*
- From:
Neuroscience Bulletin
2025;41(10):1792-1804
- CountryChina
- Language:English
-
Abstract:
Otitis media is an infection of the middle ear mainly caused by bacteria, and current treatments rely heavily on antibiotics. However, the emergence of antibiotic-resistant bacterial strains seriously affects their efficacy. In our study, we found that extracellular vesicles (EVs) derived from human natural killer cells (NKs) inhibit the proliferation of both standard and levofloxacin (LVX)-resistant strains of Staphylococcus aureus in a dose-dependent manner. Moreover, compared to LVX, EVs were more effective at reducing effusion and rescuing hearing thresholds in animal models. For LVX-sensitive strains, EVs were significantly more effective in terms of curative time but not curative rate. For LVX-resistant strains, EVs were significantly more effective in terms of both curative rate and curative time when applied alone or applied jointly with LVX. In summary, we found that NK EVs are highly effective in treating otitis media, providing an alternative approach for treating this common disease.
