Engineered Extracellular Vesicles Loaded with MiR-100-5p Antagonist Selectively Target the Lesioned Region to Promote Recovery from Brain Damage.
10.1007/s12264-025-01376-6
- Author:
Yahong CHENG
1
;
Chengcheng GAI
1
;
Yijing ZHAO
1
;
Tingting LI
1
;
Yan SONG
1
;
Qian LUO
2
;
Danqing XIN
1
;
Zige JIANG
1
;
Wenqiang CHEN
3
;
Dexiang LIU
4
;
Zhen WANG
5
Author Information
1. Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.
2. Department of Medical Psychology and Ethics, School of Basic Medicine Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.
3. Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.
4. Department of Medical Psychology and Ethics, School of Basic Medicine Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. liudexiang@sdu.edu.cn.
5. Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. wangzhen@sdu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Extracellular vesicles;
MicroRNAs;
Neonatal hypoxic-ischemic brain damage;
Neuronal survival;
Protein phosphatase 3 catalytic subunit alpha
- MeSH:
Animals;
MicroRNAs/metabolism*;
Extracellular Vesicles/metabolism*;
Mice;
Recovery of Function/physiology*;
Hypoxia-Ischemia, Brain/therapy*;
Mice, Inbred C57BL;
Antagomirs/administration & dosage*;
Male;
Animals, Newborn;
Apoptosis/drug effects*;
Brain Injuries/metabolism*;
Glycoproteins;
Peptide Fragments;
Viral Proteins
- From:
Neuroscience Bulletin
2025;41(6):1021-1040
- CountryChina
- Language:English
-
Abstract:
Hypoxic-ischemic (HI) brain damage poses a high risk of death or lifelong disability, yet effective treatments remain elusive. Here, we demonstrated that miR-100-5p levels in the lesioned cortex increased after HI insult in neonatal mice. Knockdown of miR-100-5p expression in the brain attenuated brain injury and promoted functional recovery, through inhibiting the cleaved-caspase-3 level, microglia activation, and the release of proinflammation cytokines following HI injury. Engineered extracellular vesicles (EVs) containing neuron-targeting rabies virus glycoprotein (RVG) and miR-100-5p antagonists (RVG-EVs-Antagomir) selectively targeted brain lesions and reduced miR-100-5p levels after intranasal delivery. Both pre- and post-HI administration showed therapeutic benefits. Mechanistically, we identified protein phosphatase 3 catalytic subunit alpha (Ppp3ca) as a novel candidate target gene of miR-100-5p, inhibiting c-Fos expression and neuronal apoptosis following HI insult. In conclusion, our non-invasive method using engineered EVs to deliver miR-100-5p antagomirs to the brain significantly improves functional recovery after HI injury by targeting Ppp3ca to suppress neuronal apoptosis.
