Link Brain-Wide Projectome to Neuronal Dynamics in the Mouse Brain.
10.1007/s12264-024-01232-z
- Author:
Xiang LI
1
;
Yun DU
2
;
Jiang-Feng HUANG
1
;
Wen-Wei LI
1
;
Wei SONG
2
;
Ruo-Nan FAN
1
;
Hua ZHOU
2
;
Tao JIANG
3
;
Chang-Geng LU
1
;
Zhuang GUAN
1
;
Xiao-Fei WANG
2
;
Hui GONG
1
;
Xiang-Ning LI
3
;
Anan LI
1
;
Ling FU
4
;
Yan-Gang SUN
5
Author Information
1. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, China.
2. Institute of Neuroscience, Key Laboratory of Brain Coginition and Brain-inspired Intelligence Technology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
3. HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, 215123, China.
4. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, China. lfu@mail.hust.edu.cn.
5. Institute of Neuroscience, Key Laboratory of Brain Coginition and Brain-inspired Intelligence Technology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China. yangang.sun@ion.ac.cn.
- Publication Type:Journal Article
- Keywords:
In vivo imaging;
AO imaging;
Deep brain regions;
fMOST
- MeSH:
Animals;
Neurons/physiology*;
Mice;
Brain/physiology*;
Mice, Inbred C57BL;
Somatosensory Cortex/physiology*;
Neural Pathways/physiology*;
Hippocampus/physiology*;
Mice, Transgenic;
Male;
Brain Mapping;
Nerve Net/physiology*;
Substantia Nigra/physiology*;
Tomography, Optical/methods*
- From:
Neuroscience Bulletin
2024;40(11):1621-1634
- CountryChina
- Language:English
-
Abstract:
Knowledge about the neuronal dynamics and the projectome are both essential for understanding how the neuronal network functions in concert. However, it remains challenging to obtain the neural activity and the brain-wide projectome for the same neurons, especially for neurons in subcortical brain regions. Here, by combining in vivo microscopy and high-definition fluorescence micro-optical sectioning tomography, we have developed strategies for mapping the brain-wide projectome of functionally relevant neurons in the somatosensory cortex, the dorsal hippocampus, and the substantia nigra pars compacta. More importantly, we also developed a strategy to achieve acquiring the neural dynamic and brain-wide projectome of the molecularly defined neuronal subtype. The strategies developed in this study solved the essential problem of linking brain-wide projectome to neuronal dynamics for neurons in subcortical structures and provided valuable approaches for understanding how the brain is functionally organized via intricate connectivity patterns.
