Lactobacillus plantarum ZG03 alleviates oxidative stress via its metabolites short-chain fatty acids.
10.12122/j.issn.1673-4254.2025.10.18
- Author:
Shuxian LIN
1
;
Lina GUO
1
;
Yan MA
2
;
Yao XIONG
3
;
Yingxi HE
1
;
Xinzhu XU
1
;
Wen SHENG
1
;
Suhua XU
1
;
Feng QIU
4
Author Information
1. Department of Clinical Nutrition, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China.
2. Research and Development Center, Guangdong Longseek Testing Co., Ltd., Guangzhou 510700, China.
3. Guangdong Longsee Biomedical Co., Ltd., Guangzhou 510700, China.
4. Department of Laboratory Medicine, Seventh Affiliated Hospital of Southern Medical University, Foshan 528244, China.
- Publication Type:Journal Article
- Keywords:
Lactobacillus plantarum ZG03;
glucose;
oxidative stress;
short-chain fatty acids
- MeSH:
Animals;
Zebrafish/metabolism*;
Oxidative Stress;
Lactobacillus plantarum/metabolism*;
Fatty Acids, Volatile/metabolism*;
Probiotics;
Reactive Oxygen Species/metabolism*;
Superoxide Dismutase/metabolism*
- From:
Journal of Southern Medical University
2025;45(10):2223-2230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the efficacy of Lactobacillus plantarum ZG03 (L. plantarum ZG03) for ameliorating oxidative stress in zebrafish.
METHODS:We evaluated the growth pattern of L. plantarum ZG03, observed its morphology using field emission scanning electron microscopy, and assessed its safety and potential efficacy with whole-genome sequencing for genetic analysis. FITC-labeled ZG03 was used to observe its intestinal colonization in zebrafish. In a zebrafish model of 2% glucose-induced oxidative stress, the effect of ZG03 was evaluated by assessing the changes in neutrophils in the caudal hematopoietic tissue (CHT), superoxide dismutase (SOD) activity, reactive oxygen species (ROS) levels, and malondialdehyde (MDA) content. Liquid chromatography-mass spectrometry-based targeted metabolomics was used for analyzing short-chain fatty acids (SCFAs) in the zebrafish, and the antioxidant effects of the key metabolites (acetate, propionate, and caproate) were tested.
RESULTS:On MRS agar, L. plantarum ZG03 formed circular, smooth, moist, and milky-white colonies with a rod-shaped cell morphology. Genomic analysis revealed abundant sugar metabolism gene clusters. After inoculation of FITC-labeled L. plantarum ZG03 in zebrafish, green fluorescence was clearly observed in the intestinal bulb, mid-intestine, and hind intestine. In zebrafish with glucose-induced oxidative stress, L. plantarum ZG03 significantly reduced ROS levels and the number of neutrophils in the CHT with increased SOD activity. L.plantarum ZG03 significantly increased the content of SCFAs including acetic acid, propionic acid, and caproic acid in zebrafish metabolites. In addition, sodium acetate, sodium propionate, and sodium caproate in the SCFAs significantly increased SOD activity in the zebrafish models.
CONCLUSIONS:L. plantarum ZG03 ameliorates oxidative stress in a glucose-induced zebrafish model through its metabolites, particularly the SCFAs including acetic acid, propionic acid and caproic acid.
