LINC00261 suppresses esophageal squamous cell carcinoma proliferation, invasion, and metastasis by targeting the miR-23a-3p/ZNF292 axis.
10.12122/j.issn.1673-4254.2025.10.08
- Author:
Yuan MI
1
;
Xuzhe LI
2
;
Zhanpeng WANG
3
;
Yanjie LIU
4
;
Chuntao SONG
1
;
Lantao WANG
1
;
Lei WANG
4
Author Information
1. Department of Emergency Medicine,the Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China.
2. Department of Clinical Medicine, School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050000, China.
3. Department of Emergency Medicine, Gucheng County Hospital, Hengshui 053000, China.
4. Department of Thoracic Surgery,the Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China.
- Publication Type:Journal Article
- Keywords:
LINC00261;
ZNF292;
esophageal squamous cell carcinoma;
miR-23a-3p
- MeSH:
Humans;
MicroRNAs/genetics*;
Cell Proliferation;
Esophageal Neoplasms/genetics*;
Animals;
Esophageal Squamous Cell Carcinoma;
Mice, Nude;
RNA, Long Noncoding/genetics*;
Cell Line, Tumor;
Neoplasm Invasiveness;
Mice;
Carcinoma, Squamous Cell/genetics*;
Apoptosis;
Gene Expression Regulation, Neoplastic;
Neoplasm Metastasis
- From:
Journal of Southern Medical University
2025;45(10):2118-2125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To evaluate the regulatory effects of lncRNA LINC00261 on proliferation, invasion, and metastasis of esophageal squamous cell carcinoma (ESCC) cells.
METHODS:The differentially expressed RNAs in ESCC were identified using the GSE149612 dataset from the GEO database. PCR was used to detect LINC00261 expression levels in clinical ESCC and normal esophageal tissue samples and in multiple ESCC cell lines and normal esophageal epithelial cells (HEEC). In ESCC cells, the effects of overexpression of LINC00261 on cell proliferation, invasion, metastasis and apoptosis were analyzed using CCK-8 assay, clone formation assay, Transwell assay and flow cytometry. The potential targets of LINC00261 were predicted using bioinformatics tools including ENCORI and verified using dual-luciferase reporter assay and Western blotting. The effects of LINC00261 overexpression on ESCC were confirmed in a nude mouse model bearing ESCC xenograft.
RESULTS:Analysis of the GSE149612 dataset revealed significantly lower LINC00261 expression in ESCC tissues and cell lines. In cultured ESCC cells, LINC00261 overexpression markedly suppressed cell proliferation, invasion, and metastasis and promoted cell apoptosis. Dual-luciferase reporter assays confirmed that LINC00261 targets the miR-23a-3p/ZNF292 axis. In the tumor-bearing mouse model, LINC00261 overexpression significantly inhibited ESCC xenograft proliferation and metastasis.
CONCLUSIONS:LINC00261 suppresses ESCC progression by targeting the miR-23a-3p/ZNF292 axis, suggesting a potential therapeutic strategy for ESCC treatment.
