Naoluo Xintong Decoction promotes proliferation of rat brain microvascular endothelial cells after oxygen-glucose deprivation by activating the HIF-1α/VEGF signaling pathway.
10.12122/j.issn.1673-4254.2025.09.17
- Author:
Yu ZHANG
1
;
Yinqi HU
1
;
Peipei LI
1
;
Xiao SHI
1
;
Wei XU
1
;
Jianpeng HU
1
Author Information
1. Ministry of Education Key Laboratory of Xin'an Medicine, Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
HIF-1α/VEGF signaling pathway;
Naoluo Xintong Decoction;
cerebral microvascular endothelial cells;
ischemic stroke;
oxygen-glucose deprivation/reoxygenation injury
- MeSH:
Animals;
Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*;
Drugs, Chinese Herbal/pharmacology*;
Vascular Endothelial Growth Factor A/metabolism*;
Endothelial Cells/metabolism*;
Rats;
Cell Proliferation/drug effects*;
Signal Transduction/drug effects*;
Glucose;
Brain/blood supply*;
Cells, Cultured;
Rats, Sprague-Dawley;
Vascular Endothelial Growth Factor Receptor-2/metabolism*;
Oxygen/metabolism*;
Cell Hypoxia
- From:
Journal of Southern Medical University
2025;45(9):1980-1988
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the effects of Naoluo Xintong Decoction (NLXTD) on proliferation of rat brain microvascular endothelial cells (BMECs) after oxygen-glucose deprivation/reoxygenation (OGD/R) injury and role of the HIF-1α/VEGF pathway in mediating its effect.
METHODS:Using a BMEC model of OGD/R, we tested the effects of 10% NLXTD-medicated rat serum, alone or in combination with 2ME2 or 10% NAKL, on cell proliferation, migration, tube-forming ability and permeability using CCK-8 assay, Transwell chamber assay, tube formation assay and permeability assay. Cellular expressions of VEGF and Notch were detected using ELISA and laser confocal immunofluorescence analysis, and the expressions of HIF-1α, VEGFR2, Notch1, ERK and P-ERK1/2 proteins were detected with Western blotting.
RESULTS:OGD/R injury significantly decreased viability of BMECs. NLXTD treatment of the cells with OGD/R could significantly promoted cell proliferation, migration and tube formation ability, but these effects were strongly attenuated by application of 2ME2. NLXTD treatment also significantly increased the percentages of VEGF- and Notch-positive cells in the cell models and obviously enhanced the expression levels of HIF-1α, VEGFR2, Notch1 and P-ERK1/2.
CONCLUSIONS:NLXTD promotes proliferation, migration, and tube formation of rat BMECs after OGD/R injury possibly by activating the HIF-1α/VEGF signaling pathway.
