Down-regulation of ACADM-mediated lipotoxicity inhibits invasion and metastasis of estrogen receptor-positive breast cancer cells.
10.12122/j.issn.1673-4254.2025.06.06
- Author:
Jiahao LI
1
;
Ruiting XIAN
1
;
Rong LI
1
Author Information
1. Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- Keywords:
PI3K-AKT;
breast cancer;
invasion;
lipotoxicity;
medium-chain acyl-coenzyme A dehydrogenase;
reactive oxygen species
- MeSH:
Humans;
Breast Neoplasms/metabolism*;
Female;
Mice, Nude;
Down-Regulation;
Neoplasm Invasiveness;
Animals;
Mice;
Receptors, Estrogen/metabolism*;
MCF-7 Cells;
Cell Movement;
Cell Line, Tumor;
Reactive Oxygen Species/metabolism*;
Acyl-CoA Dehydrogenase/genetics*;
Signal Transduction;
Neoplasm Metastasis;
Proto-Oncogene Proteins c-akt/metabolism*
- From:
Journal of Southern Medical University
2025;45(6):1163-1173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the effect of downregulation of medium-chain acyl-coenzyme A dehydrogenase (ACADM) on invasion and migration of estrogen receptor-positive breast cancer cells and the underlying mechanism.
METHODS:The Kaplan-Meier Plotter database was used to analyze the ACADM expression levels in breast cancer and normal tissues and their association with patient prognosis. Human breast cancer MCF-7 and T47D cell lines with lentivirus-mediated ACADM knockdown were established, and their in situ tumor formation and metastasis after tail vein injection were evaluated in nude mice. The MCF-7 and T47D cells with ACADM knockdown and their unmodified parental cells were examined with oil-red O staining assay, ROS assay, mitochondrial respiratory chain function assay before and after treatments with ROS scavenger, Elamipretide (a cardiolipin oxidation inhibitor) or SC79 (an AKT activator), and the changes in migration and invasion abilities of the treated cells were analyzed with Transwell invasion assay and Boyden chamber assay. Western blotting was used to detect protein expression levels of related signaling pathways in the treated cells.
RESULTS:ACADM overexpression was associated with a significantly shorter overall survival of breast cancer patients. In MCF-7 and T47D cells, ACADM knockdown resulted in downregulation of N calnexin, vimentin, p-P13K and p-AKT proteins, increased levels of free fatty acids and reactive oxygen species, lowered activities of mitochondrial respiratory chain complex III and V, and reduced mitochondrial inner phospholipids. ACADM knockdown significantly decreased the invasive capacity of the cells, which were obviously reversed by treatment with ROS scavenger, Elamipretide, and SC79.
CONCLUSIONS:Down-regulation of ACADM inhibits migration and invasion ability of estrogen receptor-positive breast cancer cells by lowering lipotoxicity and impairing mitochondrial function through the ROS/PI3K/AKT pathway.
