Comparison of anti-inflammatory, antibacterial and analgesic activities of formulated granules versus traditional decoction of Yinqiao Powder.
10.12122/j.issn.1673-4254.2025.05.13
- Author:
Zhuolin GUO
1
;
Zhiheng ZHANG
1
;
Xindeng GUO
1
;
Weiwei YANG
1
;
Zhiqing LIANG
1
;
Jinying OU
1
;
Huihui CAO
1
;
Zibin LU
1
;
Linzhong YU
1
;
Junshan LIU
1
Author Information
1. Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases, Guangdong Provincial Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases,School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
- Publication Type:English Abstract
- Keywords:
Yinqiao Powder;
formulated granules of traditional Chinese medicine;
pharmacological equivalence
- MeSH:
Animals;
Mice;
Drugs, Chinese Herbal/pharmacology*;
Anti-Inflammatory Agents/pharmacology*;
Analgesics/pharmacology*;
RAW 264.7 Cells;
Zebrafish;
Anti-Bacterial Agents/pharmacology*;
Powders;
Tumor Necrosis Factor-alpha/metabolism*;
Acute Lung Injury/drug therapy*;
Interleukin-6/metabolism*;
Lipopolysaccharides
- From:
Journal of Southern Medical University
2025;45(5):1003-1012
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To compare the anti-inflammatory, antibacterial and analgesic effects of Yinqiao Powder (YQS) formulated granules and decoction.
METHODS:We first evaluated the anti-inflammatory effects of the two dosage forms of YQS in a LPS-induced RAW 264.7 cell model using RT-qPCR and Western blotting. We further constructed zebrafish models of inflammation by copper sulfate exposure, caudal fin transection, or LPS and Poly (I:C) microinjection, and evaluated anti-inflammatory effects of YQS granules and decoction by examining neutrophil aggregation and HE staining findings. In a mouse model of acute lung injury (ALI) induced by intratracheal LPS instillation, the effects of YQS gavage at 10, 15, and 20 g/kg on lung pathologies were evaluated by calculating lung wet-dry weight ratio and using HE staining, ELISA and Western blotting. The microbroth dilution method was used to evaluate the antibacterial effect of YQS. Mouse pain models established by hot plate and intraperitoneal injection of glacial acetic acid were used to evaluate the analgesic effects of YQS at 10, 15, and 20 g/kg.
RESULTS:Both YQS granules and decoction significantly reduced TNF-α, IL-6, and IL-1β expressions and p-STAT3 (Tyr 705) phosphorylation level in LPS-induced RAW 264.7 cells, and obviously inhibited neutrophil aggregation in the zebrafish models. In ALI mice, YQS granules and decoction effectively ameliorated lung injury, lowered lung wet-dry weight ratio, and reduced p-STAT3 (Tyr 705) expression and TNF-α and IL-6 levels. YQS produced obvious antibacterial effect at the doses of 15.63 and 31.25 mg/mL, and significantly reduced body torsion and increased pain threshold in the mouse pain models.
CONCLUSIONS:The two dosage forms of TQS have similar anti-inflammatory, antibacterial and analgesic effects with only differences in their inhibitory effect on TNF-α, IL-6 and IL-1β mRNA expressions in LPS-induced RAW 264.7 cells.
