Asiaticoside alleviates myocardial ischemia-reperfusion injury in rats by inhibiting NLRP3 inflammasome-mediated pyroptosis.
10.12122/j.issn.1673-4254.2025.05.10
- Author:
Fenlan BIAN
1
;
Shiyao NI
2
;
Peng ZHAO
1
;
Maonanxing QI
1
;
Bi TANG
1
;
Hongju WANG
1
;
Pinfang KANG
1
;
Jinjun LIU
1
Author Information
1. Department of Cardiology , First Affiliated Hospital of Bengbu MedicalUniversity, Bengbu 233000, China.
2. Department of Otolaryngology, First Affiliated Hospital of Bengbu MedicalUniversity, Bengbu 233000, China.
- Publication Type:Journal Article
- Keywords:
NLRP3;
asiaticoside;
myocardial ischemia-reperfusion injury;
pyroptosis
- MeSH:
Animals;
Myocardial Reperfusion Injury/metabolism*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Pyroptosis/drug effects*;
Rats, Sprague-Dawley;
Rats;
Inflammasomes/metabolism*;
Triterpenes/pharmacology*;
Interleukin-18/metabolism*;
Male;
Interleukin-1beta/metabolism*;
Caspase 1/metabolism*
- From:
Journal of Southern Medical University
2025;45(5):977-985
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study the mechanism mediating the protective effect of asiaticoside (AS) against myocardial ischemia-reperfusion injury (MIRI) in rats.
METHODS:Fifty SD rats were randomized into sham-operated group, MIRI model group and AS treatment group. AS treatment was administered at low, moderate and high doses by daily gavage for 2 weeks before MIRI modeling (n=10). Serum levels of lactate dehydrogenase (LDH), creatine kinase isoenzyme (CK-MB), interleukin-18 (IL-18) and IL-1β, the volume of myocardial infarction and ischemia, and myocardial pathologies of the rats were determined or observed. The protein expression levels of NLRP3, ASC, caspase-1, GSDMD, GSDMD-N, IL-1β and IL-18 in the myocardial tissues were detected using Western blotting. The changes in the expression levels of these proteins were also detected in H9C2 cells with AS pretreatment prior to hypoxia-reoxygenation (H/R) injury.
RESULTS:The rats models of MIRI exhibited significant myocardial infarction and ischemia with increased serum levels of LDH and CK-MB and myocardial expressions of NLRP3, ASC, caspase-1, GSDMD, GSDMD-N, IL-1β and IL-18. AS pretreatment effectively reduced myocardial infarction volume in the rat models and significantly reduced serum LDH and CK-MB levels and the protein levels in the myocardial tissue in a dose-dependent manner. In the H9C2 cell model of H/R injury, AS pretreatment significantly suppressed the elevation of the protein expressions of NLRP3, ASC, caspase-1, GSDMD, GSDMD-N, IL-1β and IL-18. Molecular docking studies showed that AS had a strong binding affinity with NLRP3.
CONCLUSIONS:Asiaticoside can alleviate MIRI in rats possibly by inhibiting NLRP3 inflammasome-mediated pyroptosis.
