Cannabidiol regulates circadian rhythm to improve sleep disorders following general anesthesia in rats.
10.12122/j.issn.1673-4254.2025.04.09
- Author:
Xinshun WU
1
;
Jingcao LI
2
;
Ying LIU
2
;
Renhong QIU
2
;
Henglin WANG
3
;
Rui XYE
2
;
Yang ZHANG
2
;
Shuo LI
2
;
Qiongyin FAN
2
;
Huajin DONG
2
;
Youzhi ZHANG
2
;
Jiangbei CAO
4
Author Information
1. Medical School of Chinese PLA, Beijing 100853, China.
2. Academy of Military Medical Siences, Beijing 100850, China.
3. Department of Anesthesiology, Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048, China.
4. Department of Anesthesiology, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- Keywords:
cannabidiol;
circadian clock proteins;
postoperative sleep disturbance;
propofol
- MeSH:
Animals;
Rats, Sprague-Dawley;
Male;
Cannabidiol/therapeutic use*;
Rats;
Circadian Rhythm/drug effects*;
Propofol/adverse effects*;
Anesthesia, General/adverse effects*;
Sleep Wake Disorders/chemically induced*;
Hypothalamus/metabolism*;
Electroencephalography
- From:
Journal of Southern Medical University
2025;45(4):744-750
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To assess the regulatory effect of cannabidiol (CBD) on circadian rhythm sleep disorders following general anesthesia and explore its potential mechanism in a rat model of propofol-induced rhythm sleep disorder.
METHODS:An electrode was embedded in the skull for cortical EEG recording in 24 male SD rats, which were randomized into control, propofol, CBD treatment, and diazepam treatment groups (n=6). Eight days later, a single dose of propofol (10 mg/kg) was injected via the tail vein with anesthesia maintenance for 3 h in the latter 3 groups, and daily treatment with saline, CBD or diazepam was administered via gavage; the control rats received only saline injection. A wireless system was used for collecting EEG, EMG, and body temperature data within 72 h after propofol injection. After data collection, blood samples and hypothalamic tissue samples were collected for determining serum levels of oxidative stress markers and hypothalamic expressions of the key clock proteins.
RESULTS:Compared with the control rats, the rats with CBD treatment showed significantly increased sleep time at night (20:00-6:00), especially during the time period of 4:00-6:00 am. Compared with the rats in propofol group, which had prolonged SWS time and increased sleep episodes during 18:00-24:00 and sleep-wake transitions, the CBD-treated rats exhibited a significant reduction of SWS time and fewer SWS-to-active-awake transitions with increased SWS aspects and sleep-wake transitions at night (24:00-08:00). Diazepam treatment produced similar effect to CBD but with a weaker effect on sleep-wake transitions. Propofol caused significant changes in protein expressions and redox state, which were effectively reversed by CBD treatment.
CONCLUSIONS:CBD can improve sleep structure and circadian rhythm in rats with propofol-induced sleep disorder possibly by regulating hypothalamic expressions of the key circadian clock proteins, suggesting a new treatment option for perioperative sleep disorders.
