Protective effect of graphene heating film far-infrared hyperthermia against frostbite in mice.
10.12122/j.issn.1673-4254.2025.03.10
- Author:
Jinshui ZHANG
1
;
Shuo LI
2
;
Dongdong WEI
2
;
Xin CHENG
2
;
Yun DENG
1
;
Youzhi ZHANG
2
Author Information
1. The First Affiliated Hospital of Anhui University of Science and Technology (Huainan First People's Hospital), Huainan 232007, China.
2. Academy of Military Medical Sciences, National Key Laboratory of National Security Essential Drugs, Beijing 100850, China.
- Publication Type:Journal Article
- Keywords:
far-infrared therapy;
frostbite;
graphene heating film;
inflammation;
microcirculation
- MeSH:
Animals;
Frostbite/therapy*;
Graphite;
Mice, Inbred C57BL;
Mice;
Infrared Rays;
Mice, Inbred ICR;
Hyperthermia, Induced/methods*;
Male;
Microcirculation
- From:
Journal of Southern Medical University
2025;45(3):522-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the protective effects of graphene heating film far-infrared (FIR) hyperthermia therapy against frostbite in mice and its impacts on microcirculation and coagulation function.
METHODS:Seventy-six C57BL/6J mice were randomized into control, model, graphene-FIR, and carbon fiber-FIR groups. After 7-day FIR intervention (4 h/day), the mice were subjected to acute (4 ℃, 4 h) and intermittent (4 ℃, 4 h/day for 3 days) cold exposure and the changes in rectal temperature were monitored. In liquid nitrogen frostbite experiment, 24 ICR mice were divided into model, graphene-FIR, and carbon fiber-FIR groups, and after a 7-day FIR pretreatment (4 h/day), the liquid nitrogen frostbite models were established and apparent scores of the wounds were assessed on days 3 and 6 after modeling. In carrageenan-induced thrombosis experiment, 40 ICR mice were allocated to control, model, graphene-FIR, carbon fiber-FIR, and prazosin groups to test the effect of a 7-day FIR intervention on thrombosis induced by intraperitoneal carrageenan injection (2.5 mg/kg) by measuring thrombus length, blood perfusion, and serum biomarkers (6-keto-PGF1α, TXB2, t-PA, IL-6, IL-1β, TNF‑α) 24 h after the injection.
RESULTS:The mice in graphene-FIR group showed significantly elevated rectal temperature in cold exposure tests. In mice with liquid nitrogen-induced frostbite, graphene-FIR treatment significantly reduced the wound scores and reduced frostbite area, producing better effects than carbon fiber. In mice with carrageenan-induced thrombosis, graphene-FIR treatment significantly decreased tail thrombosis length and thrombosis area, increased blood perfusion, lowered serum levels of TXB2, TNF-α and IL-6, and increased the levels of 6-keto-PGF1α and t-PA.
CONCLUSIONS:Graphene heating film FIR therapy can alleviate frostbite injury in mice by improving microcirculation, suppressing thrombosis and inflammatory responses, and reducing coagulation dysfunction.
