Danshen Injection inhibits peritoneal dialysis fluid-induced endothelial-mesenchymal transition in HMrSV5 cells by regulating the TGF-β/Smad signaling pathway.
10.12122/j.issn.1673-4254.2024.12.02
- Author:
Lihua YU
1
;
Jingya LI
1
;
Xiaoqi WANG
2
;
Li LI
1
;
Ya CHEN
3
;
Feiyu WANG
2
;
Kun ZHANG
2
;
Tongsheng WANG
1
Author Information
1. Department of Physiology and Pharmacology, School of Integrated Chinese and Western Medicine, Taihe Traditional Chinese Medicine Hospital Affiliated to Anhui University of Chinese Medicine, Taihe 236600, China.
2. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China, Taihe Traditional Chinese Medicine Hospital Affiliated to Anhui University of Chinese Medicine, Taihe 236600, China.
3. Department of Nephrology, Taihe Traditional Chinese Medicine Hospital Affiliated to Anhui University of Chinese Medicine, Taihe 236600, China.
- Publication Type:Journal Article
- Keywords:
Danshen Injection;
TGF‑β/Smad signaling pathway;
endothelial-mesenchymal transition;
peritoneal dialysate;
peritoneal fibrosis
- MeSH:
Signal Transduction/drug effects*;
Drugs, Chinese Herbal/pharmacology*;
Transforming Growth Factor beta/metabolism*;
Humans;
Peritoneal Dialysis/adverse effects*;
Salvia miltiorrhiza;
Epithelial-Mesenchymal Transition/drug effects*;
Smad Proteins/metabolism*;
Vascular Endothelial Growth Factor A/metabolism*;
Cell Line;
Tumor Necrosis Factor-alpha/metabolism*;
Interleukin-6/metabolism*;
Cadherins/metabolism*;
Actins/metabolism*;
Dialysis Solutions;
Endothelial-Mesenchymal Transition
- From:
Journal of Southern Medical University
2024;44(12):2276-2282
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the inhibitory effect of Danshen Injection on endothelial-mesenchymal transition (EndMT) induced by peritoneal dialysis fluid in HMrSV5 cells and the role of the TGF‑β/Smad signaling pathway in mediating this effect.
METHODS:HMrSV5 cells cultured in 40% peritoneal dialysis solution for 72 h to induce EndMT were treated with 0.05%, 0.1% and 0.5% Danshen Injection. CCK-8 assay was used to assess the changes in viability of the treated cells, and the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and vascular endothelial growth factor (VEGF) in the cell supernatant were detected using ELISA; Western blotting was performed to detect the protein expressions of E-cadherin, α-smooth muscle actin (α-SMA), p-Smad 2/3, and Smad 7 in the cells.
RESULTS:Culture in 40% peritoneal dialysis fluid for 72 induced significant EndMT in HMrSV5 cells, which exhibited obviously lowered cell viability. Danshen Injection within the concentration range of 0.025%-1.5% did not significantly affect the viability of the cells. Exposure of HMrSV5 cells to peritoneal dialysis fluid for 72 h significantly increased the production of IL-6, TNF‑α, TGF‑β and VEGF, upregulated the protein expressions of α‑SMA and p-Smad 2/3, and lowered the expressions of E-cadherin and Smad7 proteins. Treatment of the exposed cells with Danshen injection significantly increased cell viability and cellular expressions of E-cadherin and Smad 7 proteins and reduced the production of IL-6, TNF-α, TGF-β and VEGF and the protein expressions of α‑SMA and p-Smad 2/3.
CONCLUSIONS:Danshen Injection can suppress peritoneal dialysis fluid-induced EndMT in HMrSV5 cells possibly by regulating the TGF-β/Smad signaling pathway.
