Alginate lyase immobilized Chlamydomonas algae microrobots: minimally invasive therapy for biofilm penetration and eradication.
10.1016/j.apsb.2025.03.034
- Author:
Xiaoting ZHANG
1
;
Huaan LI
2
;
Lu LIU
1
;
Yanzhen SONG
1
;
Lishan ZHANG
1
;
Jiajun MIAO
1
;
Jiamiao JIANG
1
;
Hao TIAN
1
;
Chang LIU
3
;
Fei PENG
4
;
Yingfeng TU
1
Author Information
1. NMPA Key Laboratory for Research and Evaluation of Drug Metabolism & Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
2. Guangdong Provincial Key Laboratory for Research and Evaluation of Pharmaceutical Preparations & Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, China.
3. Sport Science College, Beijing Sport University, Beijing 100091, China.
4. School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
- Publication Type:Journal Article
- Keywords:
Alginate lyase;
Antibacterial therapy;
Biofilms;
Biological orthogonal reaction;
Chlamydomonas reinhardtii;
Microalgae;
Microrobots;
Photodynamic therapy
- From:
Acta Pharmaceutica Sinica B
2025;15(6):3259-3272
- CountryChina
- Language:English
-
Abstract:
Bacterial biofilms can make traditional antibiotics impenetrable and even promote the development of antibiotic-resistant strains. Therefore, non-antibiotic strategies to effectively penetrate and eradicate the formed biofilms are urgently needed. Here, we demonstrate the development of self-propelled biohybrid microrobots that can enhance the degradation and penetration effects for Pseudomonas aeruginosa biofilms in minimally invasive strategy. The biohybrid microrobots (CR@Alg) are constructed by surface modification of Chlamydomonas reinhardtii (CR) microalgae with alginate lyase (Alg) via biological orthogonal reaction. By degrading the biofilm components, the number of CR@Alg microrobots with fast-moving capability penetrating the biofilm increases by around 2.4-fold compared to that of microalgae. Massive reactive oxygen species are subsequently generated under laser irradiation due to the presence of chlorophyll, inherent photosensitizers of microalgae, thus triggering photodynamic therapy (PDT) to combat bacteria. Our algae-based microrobots with superior biocompatibility eliminate biofilm-infections efficiently and tend to suppress the inflammatory response in vivo, showing huge promise for the active treatment of biofilm-associated infections.
