Prodrug-based combinational nanomedicine remodels lipid metabolism for reinforced ferroptosis and immune activation.
10.1016/j.apsb.2025.03.016
- Author:
Ling LIN
1
;
Zaixiang FANG
1
;
Guohao LIU
1
;
Yiwei LIU
1
;
Zhiqian LI
1
;
Dayi PAN
1
;
Yunkun LI
1
;
Hemi KANG
2
;
Xiaoding SHEN
1
;
Jingyao ZHANG
1
;
Qiyong GONG
1
;
Kui LUO
1
;
Jing JING
1
Author Information
1. Department of Radiology, Huaxi MR Research Center (HMRRC), Institution of Radiology and Medical Imaging, Breast Center, Institute of Breast Health Medicine, State Key Laboratory of Biotherapy, Core Facilities, West China Hospital, Sichuan University, Chengdu 610041, China.
2. College of Chemical Engineering, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Combinational therapy;
Ferroptosis;
Heparin;
Immune activation;
Lipid metabolism;
Prodrug-based nanomedicines;
ROS-responsiveness
- From:
Acta Pharmaceutica Sinica B
2025;15(5):2746-2763
- CountryChina
- Language:English
-
Abstract:
Ferroptosis is a form of programmed cell death characterized by overwhelmed lipid oxidation, and it has emerged as a promising strategy for cancer therapy. Enhanced ferroptosis could overcome the limitations of conventional therapeutic modalities, particularly in difficult-to-treat tumors. In this study, we developed a dual-modality therapy in nanomedicine by combining paclitaxel (PTX) chemotherapy and pyropheophorbide-a (Ppa) phototherapy. Heparin (HP) was grafted with poly(N-(2'-hydroxy) propyl methacrylamide) (pHPMA) using reversible addition-fragmentation chain transfer polymerization to form HP-pHPMA (HH), which was utilized to deliver Ppa and PTX, yielding HP-pHPMA-Ppa (HH-Ppa) and HP-pHPMA-PTX (HH-PTX), respectively. The prodrug-based combinational nanomedicine (HH-PP) was formed by co-assembly of HH-PTX and HH-Ppa. It was found that HH-PP treatment significantly disrupted lipid metabolism in triple-negative breast cancer (TNBC) cells, induced extensive lipid oxidation, and promoted ferroptosis. In vivo, HH-PP intervention achieved a tumor growth inhibition rate of 86.63% and activated adaptive immunity with an elevated CD8+ cytotoxic T cell infiltration level. This combinational nanomedicine offers a promising platform for co-delivery of multiple therapeutic agents. It exerts a promising anti-tumor effect via enhanced ferroptosis and ferroptosis-induced immune activation by disrupting lipid metabolism in TNBC cancer cells.
