The study on effect of EIF3B in laryngeal carcinoma.
10.13201/j.issn.2096-7993.2025.08.007
- Author:
Jie TAN
1
;
Yuguang WANG
1
;
Lin WANG
1
;
Xingguo ZHAO
1
;
Xueshi LI
1
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery，Peking University People's Hospital，Beijing，100044，China.
- Publication Type:Journal Article
- Keywords:
EIF3B;
apoptosis;
cell invasion;
laryngeal cancer
- MeSH:
Laryngeal Neoplasms/pathology*;
Humans;
Eukaryotic Initiation Factor-3/metabolism*;
Animals;
Mice, Nude;
Mice;
Cell Line, Tumor;
Cell Proliferation;
Apoptosis;
Cell Movement;
Neoplasm Staging;
Male;
Transfection;
Female;
Middle Aged;
Gene Expression Regulation, Neoplastic
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2025;39(8):729-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of EIF3B and its role in the development of laryngeal carcinoma. Methods:Immunohistochemistry, cell culture, cell transfection, qRT-PCR, Western Blot and other techniques were used to determine the expression difference of EIF3B in laryngeal cancer and adjacent tissues, and analyze the relationship between EIF3B and the size and TNM stage of laryngeal cancer. By constructing a laryngeal carcinoma cell model with EIF3B knocked down, the cell function was studied, and the regulatory effect of EIF3B on laryngeal carcinoma cells was proved in vitro. Finally, the effect of EIF3B on laryngeal carcinoma growth in vivo was studied by subcutaneous xenograft tumor model in nude mice. Results:The signal intensity of EIF3B in laryngeal carcinoma tissues was significantly stronger than that in adjacent tissues, and the expression level of EIF3B was positively correlated with patient age, TNM stage, lymph node metastasis, tumor size and clinical stage. Knocking down EIF3B can significantly inhibit the proliferation, migration and aggregation of cancer cells, and promote apoptosis. In vivo experiments with nude mice also showed that down-regulating EIF3B expression could inhibit tumor formation in vivo. Conclusion:The expression of EIF3B in laryngeal cancer is significantly increased, and it is closely related to the pathological characteristics of laryngeal cancer, which can be used as a diagnostic index of laryngeal cancer. In terms of function, EIF3B knockdown can inhibit the proliferation, migration and tumor formation of laryngeal cancer cells in vitro and in vivo, and may become a candidate target for targeted therapy of laryngeal cancer in the future.
