Exploring local microbial communities in adenoids through 16S rRNA gene sequencing.
10.13201/j.issn.2096-7993.2025.01.011
- Author:
Luohua YUAN
1
;
Haibing LIU
2
;
Wenli LI
2
;
Zhonghua PENG
2
;
Yuling MA
2
;
Jian ZOU
1
Author Information
1. Department of Otolaryngology Head and Neck Surgery，West China Hospital，Sichuan University，Chengdu，610000，China.
2. Otolaryngology Department of Sichuan Maternal and Child Health Hospital.
- Publication Type:Journal Article
- Keywords:
16S rRNA;
adenoid;
microbiota
- MeSH:
Humans;
Adenoids/microbiology*;
RNA, Ribosomal, 16S/genetics*;
Microbiota/genetics*;
Streptococcus pneumoniae/isolation & purification*;
Bacillus subtilis/genetics*;
DNA, Bacterial/analysis*
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2025;39(1):51-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the hypothesis of "pathogen storage pool" by analyzing the local microbial community of adenoids. Methods:Under the guidance of a 70° nasal endoscope, sterile swabs were used to collect secretions from the adenoid crypts of the subjects. The samples were sent to the laboratory for DNA extraction and standard bacterial 16S full-length sequencing analysis. Results:At the species level, the top three microbial communities in adenoid crypts were Bacillus subtilis（18.78%）, Fusobacterium pyogenes（11.42%）, and Streptococcus pneumoniae（9.38%）. Conclusion:The local microbial community of adenoids exhibits a high degree of diversity, including microbial communities from the oral cavity and gastrointestinal tract. Our research results support the hypothesis that adenoids act as a " pathogen reservoir".
