Improvement of neutral protease activity of <i>Bacillus amyloliquefaciens</i> LX-6 by combined ribosome engineering and medium optimization and its application in soybean meal fermentation.

Yifan ZHU; Xinyi HUANG; Tao HAN; Jiteng WANG; Xiaoping YU; Zheng MA

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Improvement of neutral protease activity of Bacillus amyloliquefaciens LX-6 by combined ribosome engineering and medium optimization and its application in soybean meal fermentation.

Author: Yifan ZHU ¹ ; Xinyi HUANG ¹ ; Tao HAN ² ; Jiteng WANG ³ ; Xiaoping YU ¹ ; Zheng MA ^{4
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Author Information

1. Key Laboratory of Microbiological Metrology, Measurement & Bio-product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
2. Department of Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China. goodhantao@gail.com.
3. Department of Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China.
4. Key Laboratory of Microbiological Metrology, Measurement & Bio-product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, China. mazheng520@
5. com.
Publication Type:Journal Article
Keywords: Bacillus amyloliquefaciens; Fermentation optimization; Neutral protease; Ribosome engineering; Soybean meal
MeSH: Bacillus amyloliquefaciens/metabolism*; Fermentation; Glycine max/metabolism*; Soybean Proteins/metabolism*; Peptide Hydrolases/metabolism*; Ribosomes/metabolism*; Globulins; Antigens, Plant; Seed Storage Proteins
From: Journal of Zhejiang University. Science. B 2025;26(8):805-812
CountryChina
Language:English
Abstract: Soybean meal (SBM) prepared by soybean crushing is the most popular protein source in the poultry and livestock industries (Cai et al., 2015) due to its economic manufacture, high protein content, and good nutritional value. Despite these benefits, SBM contains various antigen proteins such as glycinin and β-conglycinin, which account for approximately 70% of the total proteins of the SBM and reduce digestibility and damage intestinal function (Peng et al., 2018). Treating SBM with proteases (neutrase, alcalase, and trypsin) or fermentation can eliminate these antigen proteins (Contesini et al., 2018). Because of its safety and rapid growth cycle, Bacillus strains are considered ideal for the fermentation industry (Yao et al., 2021). SBM fermented by Bacillus yields products with high nutritional value and low levels of antinutritional factors (ANFs), stimulating research in this area (Yuan et al., 2017). Kumari et al. (2023) demonstrated that fermentation with Bacillus species effectively degrades antigen proteins and increases crude protein content. The degradation of antigen proteins relies on protease hydrolysis. Low protease production is the major obstacle hindering the widespread use of microbial fermentation techniques.