Establishment of a standardized daily behavior collection and analysis system for brain disease models of rhesus and cynomolgus monkeys and its application in autism spectrum disorder.

Xiaofeng REN; Huimin WANG; Xiaoman LV; Yi ZHOU; Yingyin FAN; Yanjun YU; Christoph W TURCK; Yuhui CHEN; Longbao LV; Yingzhou HU; Hao LI; Wenchao WANG; Dongdong QIN; Xiaoli FENG; Xintian HU

Return

Establishment of a standardized daily behavior collection and analysis system for brain disease models of rhesus and cynomolgus monkeys and its application in autism spectrum disorder.

Author: Xiaofeng REN ¹ ; Huimin WANG ² ; Xiaoman LV ³ ; Yi ZHOU ² ; Yingyin FAN ¹ ; Yanjun YU ¹ ; Christoph W TURCK ¹ ; Yuhui CHEN ² ; Longbao LV ¹ ; Yingzhou HU ⁴ ; Hao LI ⁵ ; Wenchao WANG ⁶ ; Dongdong QIN ^{7
,

8} ; Xiaoli FENG ^{8
,

9} ; Xintian HU ¹⁰
Author Information

1. National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Key Laboratory of Genetic Evolution & Animal Models, National Resource Center for Non-human Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650107, China.
2. Department of Physiology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, China.
3. Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China.
4. National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Key Laboratory of Genetic Evolution & Animal Models, National Resource Center for Non-human Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650107, China. xthu@mail.kiz.ac.cn, huyingzhou@mail.kiz.ac.cn.
5. National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Key Laboratory of Genetic Evolution & Animal Models, National Resource Center for Non-human Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650107, China. xthu@mail.kiz.ac.cn, lihao@mail.kiz.ac.cn.
6. National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Key Laboratory of Genetic Evolution & Animal Models, National Resource Center for Non-human Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650107, China. xthu@mail.kiz.ac.cn, wangwenchao@mail.kiz.ac.cn.
7. Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. xthu@mail.kiz.ac.cn, qindong108@
8. com.
9. Department of Physiology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, China. xthu@mail.kiz.ac.cn, virgo1985712@
10. National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Key Laboratory of Genetic Evolution & Animal Models, National Resource Center for Non-human Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650107, China. xthu@mail.kiz.ac.cn.
Publication Type:Journal Article
Keywords: Autism spectrum disorder (ASD); Cynomolgus monkey; Monkey behavior collection protocol; Monkey daily behavior ethogram; Rhesus monkey
MeSH: Animals; Autism Spectrum Disorder; Macaca mulatta; Disease Models, Animal; Behavior, Animal; Macaca fascicularis; Male; Humans
From: Journal of Zhejiang University. Science. B 2024;25(11):972-995
CountryChina
Language:English
Abstract: Complex brain diseases seriously endanger human health, and early diagnostic biomarkers and effective treatments are currently lacking. Due to ethical constraints on human research, establishing monkey models is crucial to address these issues. With the rapid development of technology, transgenic monkey models of a range of brain diseases, especially autism spectrum disorder (ASD), have been successfully established. However, to establish practical and effective brain disease models and subsequently apply them to disease mechanism and treatment studies, there is still a lack of a standard tool, i.e., a system for collecting and analyzing the daily behaviors of brain disease model monkeys. Therefore, with the goal of undertaking a comprehensive and quantitative study of behavioral phenotypes, we established a standard daily behavior collection and analysis system, including behavioral data collection protocols and a monkey daily behavior ethogram (MDBE) for rhesus and cynomolgus monkeys, which are the most commonly used non-human primates in model construction. Then, we used ASD as an application example after referring to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (DSM-5-TR), which is widely used in clinical disease diagnosis to obtain ASD core clinical symptoms. We then established a sub-ethogram (ASD monkey core behavior ethogram (MCBE-ASD)) specifically for quantitative assessment of the core clinical symptoms of an ASD monkey model based on MDBE. Subsequently, we demonstrated the high reproducibility of the system.