Cytotoxic effects of the novel photosensitizer PEG-MTPABZ-PyC-mediated photodynamic therapy on gastric cancer cells.
10.11817/j.issn.1672-7347.2025.240601
- Author:
Lingjuan CHEN
1
,
2
;
Qi WANG
3
;
Lu WANG
4
;
Yifei SHEN
3
;
Haibin WANG
3
;
Hengxin WANG
3
;
Xuejie SU
3
;
Meixu LEI
3
;
Xianxia CHEN
5
;
Chengjin AI
1
;
Yifan LI
5
;
Yali ZHOU
3
,
6
,
7
Author Information
1. Department of Laboratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu
2. cljkeyan@sina.com.
3. Cuiying Biomedical Research Center, Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou
4. Department of Nuclear Medicine, Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou
5. Department of Dermatology, Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730030, China.
6. zhouyali1956@
7. com.
- Publication Type:Journal Article
- Keywords:
PEG-MTPABZ-PyC;
cytotoxic effect;
gastric cancer;
novel photosensitizer;
photodynamic therapy
- MeSH:
Humans;
Stomach Neoplasms/drug therapy*;
Photochemotherapy/methods*;
Photosensitizing Agents/pharmacology*;
Cell Line, Tumor;
Polyethylene Glycols/chemistry*;
Reactive Oxygen Species/metabolism*;
Mesoporphyrins/pharmacology*
- From:
Journal of Central South University(Medical Sciences)
2025;50(7):1137-1144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:The application of photodynamic therapy in solid tumors has attracted increasing attention in recent years, and the efficiency of photosensitizers is a crucial determinant of therapeutic efficacy. This study aims to evaluate the cytotoxic effects of a novel photosensitizer, PEG-MTPABZ-PyC, in photodynamic therapy against gastric cancer cells.
METHODS:Gastric cancer MKN45 cells were treated with PEG-MTPABZ-PyC. A high-content live-cell imaging system was used to assess the cellular uptake kinetics and subcellular localization of the photosensitizer. The cytotoxic effects of PEG-MTPABZ-PyC-mediated photodynamic therapy were examined using the cell counting kit-8 (CCK-8) assay and flow cytometry, while the intrinsic cytotoxicity of the photosensitizer alone was verified by the CCK-8 assay. Intracellular reactive oxygen species (ROS) generation after photodynamic therapy was detected using 2'-7'-dichlorodihydrofluorescein diacetate (DCFH-DA).
RESULTS:PEG-MTPABZ-PyC alone exhibited no cytotoxicity toward MKN45 cells, indicating excellent cytocompatibility. The compound efficiently entered cells within 6 hours and localized predominantly in lysosomes. Upon light irradiation, PEG-MTPABZ-PyC-mediated photodynamic therapy induced significant cytotoxicity compared with the control group (P<0.05) and generated abundant intracellular ROS.
CONCLUSIONS:The novel photosensitizer PEG-MTPABZ-PyC demonstrates potent photodynamic cytotoxicity against gastric cancer cells, showing promising potential for further development in gastric cancer photodynamic therapy.
